Abstract
The development and maintenance of polarized epithelial tissue requires a tightly controlled orientation of mitotic cell division relative to the apical polarity axis. Hepatocytes display a unique polarized architecture. We demonstrate that mitotic hepatocytes asymmetrically segregate their apical plasma membrane domain to the nascent daughter cells. The non-polarized nascent daughter cell can form a de novo apical domain with its new neighbor. This asymmetric segregation of apical domains is facilitated by a geometrically distinct "apicolateral" subdomain of the lateral surface present in hepatocytes. The polarity protein partitioning-defective 1/microtubule-affinity regulating kinase 2 (Par1b/MARK2) translates this positional landmark to cortical polarity by promoting the apicolateral accumulation of Leu-Gly-Asn repeat-enriched protein (LGN) and the capture of nuclear mitotic apparatus protein (NuMA)-positive astral microtubules to orientate the mitotic spindle. Proliferating hepatocytes thus display an asymmetric inheritance of their apical domains via a mechanism that involves Par1b and LGN, which we postulate serves the unique tissue architecture of the developing liver parenchyma.
Original language | English |
---|---|
Article number | e1001739 |
Number of pages | 13 |
Journal | PLOS BIOLOGY |
Volume | 11 |
Issue number | 12 |
DOIs | |
Publication status | Published - 17-Dec-2013 |
Keywords
- Cell Membrane
- Cell Polarity
- Cell Proliferation
- Hep G2 Cells
- Hepatocytes
- Humans
- Intracellular Signaling Peptides and Proteins
- Metalloproteases
- Mitochondrial Proteins
- Spindle Apparatus