Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

Peter J. Deuss, Andrey A. Arzumanov, Donna L. Williams, Michael J. Gait*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)
285 Downloads (Pure)

Abstract

A novel method for the parallel synthesis of peptide-biocargo conjugates was developed that utilizes affinity purification for fast isolation of the conjugates in order to avoid time consuming HPLC purification. The methodology was applied to create two libraries of cell-penetrating peptide (CPP)-PNA705 conjugates from parallel-synthesized peptide libraries. The conjugates were tested for their ability to induce splicing redirection in HeLa pLuc705 cells. The results demonstrate how the novel methodology can be applied for screening purposes in order to find suitable CPP-biocargo combinations and further optimization of CPPs.

Original languageEnglish
Pages (from-to)7621-7630
Number of pages10
JournalOrganic & Biomolecular Chemistry
Volume11
Issue number43
DOIs
Publication statusPublished - 21-Nov-2013

Keywords

  • STERIC-BLOCK OLIGONUCLEOTIDES
  • NUCLEIC-ACIDS
  • DELIVERY

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