Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Ena Sokol; Miranda Nijenhuis; Klaas A Sjollema; Marcel F Jonkman; Hendri H Pas; Ben N G Giepmans

doi:10.1007/978-1-4939-6786-5_9

Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Ena Sokol
, Miranda Nijenhuis
, Klaas A Sjollema
, Marcel F Jonkman
, Hendri H Pas
, Ben N G Giepmans

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

4 Citations (Scopus)

Abstract

Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

Original language	English
Title of host publication	Inflammation
Subtitle of host publication	Methods and Protocols
Editors	Björn E. Clausen, Jon D. Laman
Publisher	Springer
Pages	107-118
Number of pages	12
Volume	1559
Edition	1
ISBN (Electronic)	978-1-4939-6786-5
ISBN (Print)	978-1-4939-6784-1
DOIs	https://doi.org/10.1007/978-1-4939-6786-5_9
Publication status	Published - 2017

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title = "Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins",

abstract = "Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.",

author = "Ena Sokol and Miranda Nijenhuis and Sjollema, \{Klaas A\} and Jonkman, \{Marcel F\} and Pas, \{Hendri H\} and Giepmans, \{Ben N G\}",

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doi = "10.1007/978-1-4939-6786-5\_9",

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T1 - Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

AU - Sokol, Ena

AU - Nijenhuis, Miranda

AU - Sjollema, Klaas A

AU - Jonkman, Marcel F

AU - Pas, Hendri H

AU - Giepmans, Ben N G

PY - 2017

Y1 - 2017

N2 - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

AB - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

U2 - 10.1007/978-1-4939-6786-5_9

DO - 10.1007/978-1-4939-6786-5_9

M3 - Chapter

C2 - 28063041

SN - 978-1-4939-6784-1

VL - 1559

SP - 107

EP - 118

BT - Inflammation

A2 - Clausen, Björn E.

A2 - Laman, Jon D.

PB - Springer

ER -

Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

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