Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis

Hanne-Leena Hyyrylainen, Bogumila C. Marciniak, Kathleen Dahncke, Milla Pietiainen, Pascal Courtin, Marika Vitikainen, Raili Seppala, Andreas Otto, Doerte Becher, Marie-Pierre Chapot-Chartier, Oscar P. Kuipers, Vesa P. Kontinen*, Hanne-Leena Hyyryläinen, Milla Pietiäinen

*Corresponding author for this work

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Abstract

P>The PrsA protein is a membrane-anchored peptidyl-prolyl cis-trans isomerase in Bacillus subtilis and most other Gram-positive bacteria. It catalyses the post-translocational folding of exported proteins and is essential for normal growth of B. subtilis. We studied the mechanism behind this indispensability. We could construct a viable prsA null mutant in the presence of a high concentration of magnesium. Various changes in cell morphology in the absence of PrsA suggested that PrsA is involved in the biosynthesis of the cylindrical lateral wall. Consistently, four penicillin-binding proteins (PBP2a, PBP2b, PBP3 and PBP4) were unstable in the absence of PrsA, while muropeptide analysis revealed a 2% decrease in the peptidoglycan cross-linkage index. Misfolded PBP2a was detected in PrsA-depleted cells, indicating that PrsA is required for the folding of this PBP either directly or indirectly. Furthermore, strongly increased uniform staining of cell wall with a fluorescent vancomycin was observed in the absence of PrsA. We also demonstrated that PrsA is a dimeric or oligomeric protein which is localized at distinct spots organized in a helical pattern along the cell membrane. These results suggest that PrsA is essential for normal growth most probably as PBP folding is dependent on this PPIase.

Original languageEnglish
Pages (from-to)108-127
Number of pages20
JournalMolecular Microbiology
Volume77
Issue number1
DOIs
Publication statusPublished - Jul-2010

Keywords

  • ACTIN-LIKE PROTEINS
  • REGULATED INTRAMEMBRANE PROTEOLYSIS
  • WALL-ASSOCIATED PROTEASE
  • CELL-WALL
  • SECRETION STRESS
  • PEPTIDOGLYCAN STRUCTURE
  • LACTOCOCCUS-LACTIS
  • CORYNEBACTERIUM-GLUTAMICUM
  • CAULOBACTER-CRESCENTUS
  • CIS/TRANS ISOMERASES

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