Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels

Anneke C. Muller Kobold; Ido P. Kema; Janneke Dijck-Brouwer; Karin Koerts; Michiel Weening; Twan Storteboom; Jenny E. Kootstra-Ros; Martijn van Faassen; Ineke J. Riphagen

doi:10.1515/cclm-2021-0722

Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels

Anneke C. Muller Kobold^*, Ido P. Kema, Janneke Dijck-Brouwer, Karin Koerts, Michiel Weening, Twan Storteboom, Jenny E. Kootstra-Ros, Martijn van Faassen, Ineke J. Riphagen

^*Corresponding author for this work

Research output: Contribution to journal › Letter › Academic › peer-review

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Original language	English
Pages (from-to)	e18-e20
Number of pages	3
Journal	Clinical chemistry and laboratory medicine
Volume	60
Issue number	1
DOIs	https://doi.org/10.1515/cclm-2021-0722
Publication status	Published - 1-Jan-2022

Keywords

immunoassay
liquid chromatography-tandem mass spectrometry (LC-MS/MS)
pepsin digestion
vitamin D
vitamin D-binding protein (VDBP)
CLINICAL-PERFORMANCE
LC-MS/MS
ASSAY

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Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levelsFinal publisher's version, 281 KBLicence: CC BY

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Muller Kobold, A. C., Kema, I. P., Dijck-Brouwer, J., Koerts, K., Weening, M., Storteboom, T., Kootstra-Ros, J. E., van Faassen, M., & Riphagen, I. J. (2022). Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels. Clinical chemistry and laboratory medicine, 60(1), e18-e20. https://doi.org/10.1515/cclm-2021-0722

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title = "Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels",

keywords = "immunoassay, liquid chromatography-tandem mass spectrometry (LC-MS/MS), pepsin digestion, vitamin D, vitamin D-binding protein (VDBP), CLINICAL-PERFORMANCE, LC-MS/MS, ASSAY",

author = "{Muller Kobold}, {Anneke C.} and Kema, {Ido P.} and Janneke Dijck-Brouwer and Karin Koerts and Michiel Weening and Twan Storteboom and Kootstra-Ros, {Jenny E.} and {van Faassen}, Martijn and Riphagen, {Ineke J.}",

year = "2022",

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day = "1",

doi = "10.1515/cclm-2021-0722",

language = "English",

volume = "60",

pages = "e18--e20",

journal = "Clinical chemistry and laboratory medicine",

issn = "1434-6621",

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Muller Kobold, AC , Kema, IP , Dijck-Brouwer, J, Koerts, K, Weening, M, Storteboom, T , Kootstra-Ros, JE , van Faassen, M & Riphagen, IJ 2022, 'Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels', Clinical chemistry and laboratory medicine, vol. 60, no. 1, pp. e18-e20. https://doi.org/10.1515/cclm-2021-0722

Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels. / Muller Kobold, Anneke C.; Kema, Ido P.; Dijck-Brouwer, Janneke et al.
In: Clinical chemistry and laboratory medicine, Vol. 60, No. 1, 01.01.2022, p. e18-e20.

Research output: Contribution to journal › Letter › Academic › peer-review

TY - JOUR

T1 - Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels

AU - Muller Kobold, Anneke C.

AU - Kema, Ido P.

AU - Dijck-Brouwer, Janneke

AU - Koerts, Karin

AU - Weening, Michiel

AU - Storteboom, Twan

AU - Kootstra-Ros, Jenny E.

AU - van Faassen, Martijn

AU - Riphagen, Ineke J.

PY - 2022/1/1

Y1 - 2022/1/1

KW - immunoassay

KW - liquid chromatography-tandem mass spectrometry (LC-MS/MS)

KW - pepsin digestion

KW - vitamin D

KW - vitamin D-binding protein (VDBP)

KW - CLINICAL-PERFORMANCE

KW - LC-MS/MS

KW - ASSAY

U2 - 10.1515/cclm-2021-0722

DO - 10.1515/cclm-2021-0722

M3 - Letter

SN - 1434-6621

VL - 60

SP - e18-e20

JO - Clinical chemistry and laboratory medicine

JF - Clinical chemistry and laboratory medicine

IS - 1

ER -