Peripheral blood leukocyte NO production and oxidative stress in multiple sclerosis

Marcus Koch*, Jop Mostert, Alexander Arutjunyan, Michael Stepanov, Albert Teelken, Dorothea Heersema, Jacques De Keyser

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    18 Citations (Scopus)

    Abstract

    Background The reason for increased peripheral blood leukocyte ( PBL) nitric oxide ( NO) production in patients with multiple sclerosis ( MS) is unknown.

    Objective To investigate whether PBL NO production is related to measures of oxidative stress.

    Methods PBL nitrite, diene conjugates ( DC, a measure of undergone oxidative stress), antiradical activity ( ARA) and antioxidant acitvity ( AOA) were measured in 35 healthy control persons and 80 patients with MS. We investigated the correlation of these measures with a partial correlation analysis, with age as the control variable.

    Results There was a significant correlation in both MS patients and healthy control persons between PBL nitrite levels and PBL DC, ARA and AOA. The correlations were stronger in healthy control persons. An analysis by disease subtype showed that the correlations were present in patients with relapsing - remitting and secondary progressive MS, but absent in primary progressive MS.

    Conclusions PBL nitrite levels and measures of oxidative stress are closely related in MS- patients as well as in healthy control persons. Increased serum NO levels in MS may be the result of a physiologic reaction to overall oxidative stress. The differences in the strength of correlation between different disease subtypes may reflect differences in leukocyte biology. Multiple Sclerosis 2008.

    Original languageEnglish
    Pages (from-to)159-165
    Number of pages7
    JournalMultiple Sclerosis
    Volume14
    Issue number2
    DOIs
    Publication statusPublished - Mar-2008

    Keywords

    • multiple sclerosis
    • oxidative stress
    • nitric oxide
    • leukocytes
    • NITRIC-OXIDE METABOLITES
    • NF-KAPPA-B
    • MATRIX-METALLOPROTEINASE-9 GENE-EXPRESSION
    • EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
    • CEREBROSPINAL-FLUID NITRITE
    • LIPID-PEROXIDATION PRODUCTS
    • SYNTHASE EXPRESSION
    • ACTIVITY MARKER
    • MS PATIENTS
    • SERUM

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