Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites

Huala Wu, Rinse de Boer, Arjen M Krikken, Arman Akşit, Wei Yuan, Ida J van der Klei*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
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Abstract

Using electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes and other cell organelles in the yeast Hansenula polymorpha. In exponential glucose-grown cells, which typically contain a single small peroxisome, contacts were only observed with the endoplasmic reticulum and the plasma membrane. Here we focus on a novel peroxisome-vacuole contact site that is formed when glucose-grown cells are shifted to methanol containing media, conditions that induce strong peroxisome development. At these conditions, the small peroxisomes rapidly increase in size, a phenomenon that is paralleled by the formation of distinct intimate contacts with the vacuole. Localization studies showed that the peroxin Pex3 accumulated in patches at the peroxisome-vacuole contact sites. In wild-type cells growing exponentially on medium containing glucose, peroxisome-vacuole contact sites were never observed. However, upon overproduction of Pex3 peroxisomes also associated to vacuoles at these growth conditions. Our observations strongly suggest a role for Pex3 in the formation of a novel peroxisome-vacuole contact site. This contact likely plays a role in membrane growth as it is formed solely at conditions of strong peroxisome expansion.

Original languageEnglish
Pages (from-to)349-359
Number of pages11
JournalBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume1866
Issue number3
DOIs
Publication statusPublished - Mar-2019

Keywords

  • Yeast
  • Peroxisome
  • vacuole
  • contact sites
  • Pex3
  • HANSENULA-POLYMORPHA
  • SELECTIVE AUTOPHAGY
  • GENE ENCODES
  • PROTEIN
  • ER
  • DEGRADATION
  • CELLS
  • PEXOPHAGY
  • GLUCOSE
  • PEX3P

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