PET Imaging with S-[C-11]Methyl-L-Cysteine and L-[Methyl-C-11]Methionine in Rat Models of Glioma, Glioma Radiotherapy, and Neuroinflammation

Andrea Parente, Aren van Waarde*, Alexandre Shoji, Daniele de Paula Faria, Bram Maas, Rolf Zijlma, Rudi Dierckx, J.A. Langendijk, Erik de Vries, Janine Doorduin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

PURPOSE: S-[(11)C]-methyl-L-cysteine ([(11)C]MCYS) has been claimed to offer higher tumor selectivity than L-[methyl- (11)C]methionine ([(11)C]MET). We examined this claim in animal models.

PROCEDURES: Rats with implanted untreated (n = 10) or irradiated (n = 7, 1 × 25 Gy, on day 8) orthotopic gliomas were scanned after 6, 9, and 12 days, using positron emission tomography. Rats with striatal injections of saline (n = 9) or bacterial lipopolysaccharide (n = 9) were scanned after 3 days.

RESULTS: Uptake of the two tracers in untreated gliomas was similar. [(11)C]MCYS was not accumulated in salivary glands, nasal epithelium, and healing wounds, in contrast to [(11)C]MET, but showed 40 % higher accumulation in the healthy brain. Both tracers showed a reduced tumor uptake 4 days after irradiation and minor accumulation in inflamed striatum. [(11)C]MCYS indicated higher lesion volumes than [(11)C]MET (untreated tumor + 47 %; irradiated tumor up to + 500 %; LPS-inflamed striatum + 240 %).

CONCLUSIONS: [(11)C]MCYS was less accumulated in some non-tumor tissues than [(11)C]MET, but showed lower tumor-to-brain contrast.

Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalMolecular Imaging and Biology
Volume20
Issue number3
Early online date30-Oct-2017
DOIs
Publication statusPublished - Jun-2018

Keywords

  • Amino acids
  • Brain tumors
  • Inflammation
  • Brain
  • Positron emission tomography
  • Small animal imaging
  • INFLAMMATION
  • TUMOR

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