TY - JOUR
T1 - PET Imaging with S-[C-11]Methyl-L-Cysteine and L-[Methyl-C-11]Methionine in Rat Models of Glioma, Glioma Radiotherapy, and Neuroinflammation
AU - Parente, Andrea
AU - van Waarde, Aren
AU - Shoji, Alexandre
AU - de Paula Faria, Daniele
AU - Maas, Bram
AU - Zijlma, Rolf
AU - Dierckx, Rudi
AU - Langendijk, J.A.
AU - de Vries, Erik
AU - Doorduin, Janine
PY - 2018/6
Y1 - 2018/6
N2 - PURPOSE: S-[(11)C]-methyl-L-cysteine ([(11)C]MCYS) has been claimed to offer higher tumor selectivity than L-[methyl- (11)C]methionine ([(11)C]MET). We examined this claim in animal models.PROCEDURES: Rats with implanted untreated (n = 10) or irradiated (n = 7, 1 × 25 Gy, on day 8) orthotopic gliomas were scanned after 6, 9, and 12 days, using positron emission tomography. Rats with striatal injections of saline (n = 9) or bacterial lipopolysaccharide (n = 9) were scanned after 3 days.RESULTS: Uptake of the two tracers in untreated gliomas was similar. [(11)C]MCYS was not accumulated in salivary glands, nasal epithelium, and healing wounds, in contrast to [(11)C]MET, but showed 40 % higher accumulation in the healthy brain. Both tracers showed a reduced tumor uptake 4 days after irradiation and minor accumulation in inflamed striatum. [(11)C]MCYS indicated higher lesion volumes than [(11)C]MET (untreated tumor + 47 %; irradiated tumor up to + 500 %; LPS-inflamed striatum + 240 %).CONCLUSIONS: [(11)C]MCYS was less accumulated in some non-tumor tissues than [(11)C]MET, but showed lower tumor-to-brain contrast.
AB - PURPOSE: S-[(11)C]-methyl-L-cysteine ([(11)C]MCYS) has been claimed to offer higher tumor selectivity than L-[methyl- (11)C]methionine ([(11)C]MET). We examined this claim in animal models.PROCEDURES: Rats with implanted untreated (n = 10) or irradiated (n = 7, 1 × 25 Gy, on day 8) orthotopic gliomas were scanned after 6, 9, and 12 days, using positron emission tomography. Rats with striatal injections of saline (n = 9) or bacterial lipopolysaccharide (n = 9) were scanned after 3 days.RESULTS: Uptake of the two tracers in untreated gliomas was similar. [(11)C]MCYS was not accumulated in salivary glands, nasal epithelium, and healing wounds, in contrast to [(11)C]MET, but showed 40 % higher accumulation in the healthy brain. Both tracers showed a reduced tumor uptake 4 days after irradiation and minor accumulation in inflamed striatum. [(11)C]MCYS indicated higher lesion volumes than [(11)C]MET (untreated tumor + 47 %; irradiated tumor up to + 500 %; LPS-inflamed striatum + 240 %).CONCLUSIONS: [(11)C]MCYS was less accumulated in some non-tumor tissues than [(11)C]MET, but showed lower tumor-to-brain contrast.
KW - Amino acids
KW - Brain tumors
KW - Inflammation
KW - Brain
KW - Positron emission tomography
KW - Small animal imaging
KW - INFLAMMATION
KW - TUMOR
U2 - 10.1007/s11307-017-1137-z
DO - 10.1007/s11307-017-1137-z
M3 - Article
C2 - 29086198
SN - 1536-1632
VL - 20
SP - 465
EP - 472
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
IS - 3
ER -