The research described in this thesis aimed to investigate how and why PMP degradation occurs. Our study focuses on the degradation of the PMP Pex13p in yeast and the corresponding function of Pex13p degradation in the context of peroxisome biology and cellular metabolism. We show that the PMP Pex13p undergoes rapid turnover in H. polymorpha wildtype cells and describe a role of the peroxisomal ubiquitination machinery in Pex13p turnover. we investigate the potential functions of Pex13p degradation in the yeast H. polymorpha. We demonstrated that Pex2p-dependent turnover of Pex13p also occurs under peroxisome non-inducing condition, demonstrating that Pex13p degradation is a general and not a condition-specific event. We also show that blocking the recycling of Pex5p inhibits Pex13p degradation, indicating that the removal of Pex5p from the peroxisomal membrane is linked to Pex13p degradation. we have investigated Pex13p degradation in the yeast S. cerevisiae and utilized a tandem fluorescent protein timer (tFT) to identify additional factors involved in Pex13p degradation. Our data demonstrate that Pex13p rapid degradation is conserved in S. cerevisiae wild type cells grown on oleic acid media and that Pex13p is degraded via UPS, again establishing that Pex13p turnover is likely to play an important role in peroxisome biology.
|Qualification||Doctor of Philosophy|
|Place of Publication||[Groningen]|
|Publication status||Published - 2019|