Phospho-Rasputin Stabilization by Sec16 Is Required for Stress Granule Formation upon Amino Acid Starvation

Angelica Aguilera-Gomez, Margarita Zacharogianni, Marinke M. van Oorschot, Heide Genau, Rianne Grond, Tineke Veenendaal, Kristina S. Sinsimer, Elisabeth A. Gavis, Christian Behrends, Catherine Rabouille*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)
    273 Downloads (Pure)

    Abstract

    Most cellular stresses induce protein translation inhibition and stress granule formation. Here, using Drosophila S2 cells, we investigate the role of G3BP/Rasputin in this process. In contrast to arsenite treatment, where dephosphorylated Ser142 Rasputin is recruited to stress granules, we find that, upon amino acid starvation, only the phosphorylated Ser142 form is recruited. Furthermore, we identify Sec16, a component of the endoplasmic reticulum exit site, as a Rasputin interactor and stabilizer. Sec16 depletion results in Rasputin degradation and inhibition of stress granule formation. However, in the absence of Sec16, pharmacological stabilization of Rasputin is not enough to rescue the assembly of stress granules. This is because Sec16 specifically interacts with phosphorylated Ser142 Rasputin, the form required for stress granule formation upon amino acid starvation. Taken together, these results demonstrate that stress granule formation is finetuned by specific signaling cues that are unique to each stress. These results also expand the role of Sec16 as a stress response protein.

    Original languageEnglish
    Pages (from-to)935-948
    Number of pages14
    JournalCell reports
    Volume20
    Issue number4
    DOIs
    Publication statusPublished - 25-Jul-2017

    Keywords

    • ER-EXIT SITES
    • ENDOPLASMIC-RETICULUM
    • SECRETORY PATHWAY
    • TRANSITIONAL ER
    • NONREDUNDANT FUNCTIONS
    • PHASE-TRANSITION
    • MAMMALIAN-CELLS
    • RNA GRANULES
    • TER SITES
    • DROSOPHILA

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