Phosphoprotein levels, MAPK activities and NF kappa B expression are affected by fisetin

Roberta R. Ruela De Sousa, Karla Cristiana Souza Queiroz, Ana Carolina Santos Souza, Sonia A. Gurgueira, Amanda Cavallari Augusto, Marcio Andre Miranda, Maikel P. Peppelenbosch, Carmen Verissima Ferreira, Hiroshi Aoyama*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    87 Citations (Scopus)

    Abstract

    Flavonoids, polyphenolic phytochemicals, are ubiquitous in plants and are commonly present in the human diet. They may exert diverse beneficial effects, including antioxidant and anticarcinogenic activities. The present study was designed to evaluate three biomolecules that play important roles in the apoptotic process: mitogen-activated protein kinases, protein phosphatases and NFkB, using HL60 cells treated with fisetin as an experimental model. Our results demonstrated that cells treated with fisetin presented high expression of NFkB, activation of MAPK p38 and an increase of phosphoprotein levels; inhibition of enzymes involved in redox status maintenance were also observed. Our findings reinforce the hypothesis that fisetin is likely to exert beneficial and/or toxic actions on cells not through its potential as antioxidant but rather through its modulation of protein kinase and phosphatase signaling cascades. Additionally, our results also indicate that the cellular effects of fisetin will ultimately depend on the cell type and on the extent to which they associate with the cells, either by interactions at the membrane or by uptake into the cytosol.

    Original languageEnglish
    Pages (from-to)439-444
    Number of pages6
    JournalJournal of enzyme inhibition and medicinal chemistry
    Volume22
    Issue number4
    DOIs
    Publication statusPublished - 2007

    Keywords

    • fisetin
    • flavonoids
    • HL60
    • NF kappa B
    • MAPK
    • phosphatases
    • DNA TOPOISOMERASE-II
    • KINASE-C INHIBITION
    • REACTIVE OXYGEN
    • INDUCED APOPTOSIS
    • IN-VITRO
    • SUPEROXIDE DISMUTASE
    • HYDROGEN-PEROXIDE
    • PLANT FLAVONOIDS
    • LEUKEMIA-CELLS
    • ACTIVATION

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