Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa

Philippe Selhorst; Sebastian Lequime; Gytis Dudas; Sam Proesmans; Pascal Lutumba; Freddy Katshongo; Kadrie Ramadan; Isabel Micalessi; Steve Ahuka-Mundeke; Veerle Vanlerberghe; Marjan Van Esbroeck; KevinK Ariën

doi:10.1016/j.ijid.2023.04.391

Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa

Philippe Selhorst
, Sebastian Lequime
, Gytis Dudas
, Sam Proesmans
, Pascal Lutumba
, Freddy Katshongo
, Kadrie Ramadan
, Isabel Micalessi
, Steve Ahuka-Mundeke
, Veerle Vanlerberghe
, Marjan Van Esbroeck
, KevinK Ariën

Lequime lab - Virus Ecology and Evolution

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

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Abstract

OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data.

METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.

RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.

CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.

Original language	English
Pages (from-to)	46-52
Number of pages	7
Journal	International Journal of Infectious Diseases
Volume	133
Early online date	22-Apr-2023
DOIs	https://doi.org/10.1016/j.ijid.2023.04.391
Publication status	Published - Aug-2023

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Selhorst, P., Lequime, S., Dudas, G., Proesmans, S., Lutumba, P., Katshongo, F., Ramadan, K., Micalessi, I., Ahuka-Mundeke, S., Vanlerberghe, V., Van Esbroeck, M., & Ariën, K. (2023). Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa. International Journal of Infectious Diseases, 133, 46-52. https://doi.org/10.1016/j.ijid.2023.04.391

@article{b9389718147f424e9410084e1e90188a,

title = "Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa",

abstract = "OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1\% of global sequence data.METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.",

author = "Philippe Selhorst and Sebastian Lequime and Gytis Dudas and Sam Proesmans and Pascal Lutumba and Freddy Katshongo and Kadrie Ramadan and Isabel Micalessi and Steve Ahuka-Mundeke and Veerle Vanlerberghe and \{Van Esbroeck\}, Marjan and KevinK Ari{\"e}n",

note = "Copyright {\textcopyright} 2023. Published by Elsevier Ltd.",

year = "2023",

month = aug,

doi = "10.1016/j.ijid.2023.04.391",

language = "English",

volume = "133",

pages = "46--52",

journal = "International Journal of Infectious Diseases",

issn = "1201-9712",

publisher = "ELSEVIER SCI LTD",

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Selhorst, P, Lequime, S, Dudas, G, Proesmans, S, Lutumba, P, Katshongo, F, Ramadan, K, Micalessi, I, Ahuka-Mundeke, S, Vanlerberghe, V, Van Esbroeck, M & Ariën, K 2023, 'Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa', International Journal of Infectious Diseases, vol. 133, pp. 46-52. https://doi.org/10.1016/j.ijid.2023.04.391

TY - JOUR

T1 - Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa

AU - Selhorst, Philippe

AU - Lequime, Sebastian

AU - Dudas, Gytis

AU - Proesmans, Sam

AU - Lutumba, Pascal

AU - Katshongo, Freddy

AU - Ramadan, Kadrie

AU - Micalessi, Isabel

AU - Ahuka-Mundeke, Steve

AU - Vanlerberghe, Veerle

AU - Van Esbroeck, Marjan

AU - Ariën, KevinK

PY - 2023/8

Y1 - 2023/8

N2 - OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data.METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.

AB - OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data.METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.

U2 - 10.1016/j.ijid.2023.04.391

DO - 10.1016/j.ijid.2023.04.391

M3 - Article

C2 - 37088357

SN - 1201-9712

VL - 133

SP - 46

EP - 52

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

ER -

Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa

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