TY - JOUR
T1 - Plasticity of the adult human small intestinal stoma microbiota
AU - Yilmaz, Bahtiyar
AU - Fuhrer, Tobias
AU - Morgenthaler, Deborah
AU - Krupka, Niklas
AU - Wang, Daoming
AU - Spari, Daniel
AU - Candinas, Daniel
AU - Misselwitz, Benjamin
AU - Beldi, Guido
AU - Sauer, Uwe
AU - Macpherson, Andrew J.
N1 - Funding Information:
We thank the staff of Inselspital for technical and financial support. We are grateful to Dr. Peter Studer and Dr. Pascal Juillerat for sample collections and Dr. Ruben Mars for metabolic sample processing. We are thankful to the University of Bern NGS Platform for metagenomic sequencing. This work was supported by the Swiss National Science Foundation ( SNF Sinergia CRSII3_154414 and SNF Sinergia CRSII5_177164 to A.J.M.; SNF Ambizione Grant PZ00P3_185880 to B.Y. and SNF Grant 320030_197815 to B.M.) A.J.M. was supported by European Research Council (HHMM-Neonates Project Number: 742195 ). A.J.M. and B.Y. were also supported by Bern Center for Precision Medicine (BCPM), University of Bern.
Funding Information:
We thank the staff of Inselspital for technical and financial support. We are grateful to Dr. Peter Studer and Dr. Pascal Juillerat for sample collections and Dr. Ruben Mars for metabolic sample processing. We are thankful to the University of Bern NGS Platform for metagenomic sequencing. This work was supported by the Swiss National Science Foundation (SNF Sinergia CRSII3_154414 and SNF Sinergia CRSII5_177164 to A.J.M.; SNF Ambizione Grant PZ00P3_185880 to B.Y. and SNF Grant 320030_197815 to B.M.) A.J.M. was supported by European Research Council (HHMM-Neonates Project Number: 742195). A.J.M. and B.Y. were also supported by Bern Center for Precision Medicine (BCPM), University of Bern. Conceptualization, A.J.M. and B.Y.; investigation, B.Y. A.J.M. D.M. T.F. N.K. D.S. D.C. G.B. B.M. and U.S.; data analysis, B.Y. A.J.M. T.F. and D.W.; resources, A.J.M.; writing – original draft, A.J.M. and B.Y.; writing – review & editing, A.J.M. and B.Y.; funding acquisition, A.J.M. B.Y. and U.S. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research.
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/12/14
Y1 - 2022/12/14
N2 - The human distal small intestine (ileum) has a distinct microbiota, but human studies investigating its composition and function have been limited by the inaccessibility of the ileum without purging and/or deep intubation. We investigated inherent instability, temporal dynamics, and the contribution of fed and fasted states using stoma samples from cured colorectal cancer patients as a non-invasive access route to the otherwise inaccessible small and large intestines. Sequential sampling of the ileum before and after stoma formation indicated that ileostoma microbiotas represented that of the intact small intestine. Ileal and colonic stoma microbiotas were confirmed as distinct, and two types of instability in ileal host-microbial relationships were observed: inter-digestive purging followed by the rapid postprandial blooming of bacterial biomass and sub-strain appearance and disappearance within individual taxa after feeding. In contrast to the relative stability of colonic microbiota, the human small intestinal microbiota biomass and its sub-strain composition can be highly dynamic.
AB - The human distal small intestine (ileum) has a distinct microbiota, but human studies investigating its composition and function have been limited by the inaccessibility of the ileum without purging and/or deep intubation. We investigated inherent instability, temporal dynamics, and the contribution of fed and fasted states using stoma samples from cured colorectal cancer patients as a non-invasive access route to the otherwise inaccessible small and large intestines. Sequential sampling of the ileum before and after stoma formation indicated that ileostoma microbiotas represented that of the intact small intestine. Ileal and colonic stoma microbiotas were confirmed as distinct, and two types of instability in ileal host-microbial relationships were observed: inter-digestive purging followed by the rapid postprandial blooming of bacterial biomass and sub-strain appearance and disappearance within individual taxa after feeding. In contrast to the relative stability of colonic microbiota, the human small intestinal microbiota biomass and its sub-strain composition can be highly dynamic.
KW - bacterial biomass
KW - colostomy
KW - ileostomy
KW - metabolomics
KW - microbial instability
KW - small intestinal microbiota
KW - sub-strains
U2 - 10.1016/j.chom.2022.10.002
DO - 10.1016/j.chom.2022.10.002
M3 - Article
C2 - 36318918
AN - SCOPUS:85143738766
SN - 1931-3128
VL - 30
SP - 1773
EP - 1787
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 12
ER -