Positioning Bacillus subtilis as terpenoid cell factory

Hegar Pramastya, Yafeng Song, Elfahmi Yaman, Sukrasno Sukrasno, Wim J Quax*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Citations (Scopus)
4 Downloads (Pure)

Abstract

Increasing demands for bioactive compounds have motivated researchers to employ micro-organisms to produce complex natural products. Currently, Bacillus subtilis has been attracting lots of attention to be developed into terpenoids cell factories due to its generally recognized safe status and high isoprene precursor biosynthesis capacity by endogenous methylerythritol phosphate (MEP) pathway. In this review, we describe the up-to-date knowledge of each enzyme in MEP pathway and the subsequent steps of isomerization and condensation of C5 isoprene precursors. In addition, several representative terpene synthases expressed in B. subtilis and the engineering steps to improve corresponding terpenoids production are systematically discussed. Furthermore, the current available genetic tools are mentioned as along with promising strategies to improve terpenoids in B. subtilis, hoping to inspire future directions in metabolic engineering of B. subtilis for further terpenoid cell factory development.

Original languageEnglish
Pages (from-to)1839-1856
Number of pages18
JournalJournal of Applied Microbiology
Volume130
Issue number6
Early online date24-Oct-2020
DOIs
Publication statusPublished - 2020

Keywords

  • Bacillus subtilis
  • cell factory
  • MEP pathway
  • metabolic engineering
  • terpenoids
  • METHYLERYTHRITOL PHOSPHATE-PATHWAY
  • II ISOPENTENYL DIPHOSPHATE
  • ISOPRENOID BIOSYNTHESIS
  • ESCHERICHIA-COLI
  • MEVALONATE PATHWAY
  • CRYSTAL-STRUCTURE
  • TAXADIENE BIOSYNTHESIS
  • 4-PHOSPHATE PATHWAY
  • FUNCTIONAL-ANALYSIS
  • SYNTHASE

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