Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases

Jeroen E. van Schaik; Bert van der Vegt; Lorian Slagter-Menkema; Saskia H. Hanemaaijer; Gyorgi B. Halmos; Max J.H. Witjes; Bernard F.A.M. van der Laan; Rudolf S.N. Fehrmann; Sjoukje F. Oosting; Boudewijn E.C. Plaat

doi:10.1016/j.amjoto.2024.104298

Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases

Jeroen E. van Schaik, Bert van der Vegt, Lorian Slagter-Menkema, Saskia H. Hanemaaijer, Gyorgi B. Halmos, Max J.H. Witjes, Bernard F.A.M. van der Laan, Rudolf S.N. Fehrmann, Sjoukje F. Oosting, Boudewijn E.C. Plaat^*

^*Corresponding author for this work

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Abstract

Purpose: To investigate glycoprotein nonmetastatic melanoma protein B (GPNMB) and vascular endothelial growth factor (VEGF) as potential fluorescent imaging markers by comparing their protein expression to epidermal growth factor receptor (EGFR).

Materials and methods: Thirty-eight paired samples of untreated head and neck squamous cell carcinoma (HNSCC) primary tumours (PT) and corresponding synchronous lymph node metastases (LNM) were selected. After immunohistochemical staining, expression was assessed and compared by the percentage of positive tumour cells. Data were analysed using the Mann-Whitney test, effect sizes (ESr) and Spearman's correlation coefficient (r).

Results: GPNMB expression was observed in 100 % of PT, and median 80 % (range 5–100 %) of tumour cells, VEGF in 92 % and 60 % (0–100 %), EGFR in 87 % and 60 % (0–100 %) respectively. In corresponding LNM, GPNMB expression was observed in 100 % of LNM and median 90 % (20–100 %) of tumour cells, VEGF in 87 % and 65 % (0–100 %), and EGFR in 84 % and 35 % (0–100 %). A positive correlation was found between expression in PT and LNM for GPNMB (r = 0.548) and EGFR (r = 0.618) (p < 0.001), but not for VEGF (r = −0.020; p = 0.905). GPNMB expression was present in a higher percentage of tumour cells compared to EGFR in PT (p = 0.015, ESr = −0.320) and in LNM (p < 0.001, ESr = −0.478), while VEGF was not (p = 1.00, ESr = −0.109 and − 0.152, respectively).

Conclusion: GPNMB expression is higher than EGFR in untreated HNSCC PT and corresponding LNM, while VEGF expression is comparable to EGFR. GPNMB is a promising target for fluorescent imaging in HNSCC.

Original language	English
Article number	104298
Number of pages	5
Journal	American Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume	45
Issue number	4
DOIs	https://doi.org/10.1016/j.amjoto.2024.104298
Publication status	Published - 1-Jul-2024

Keywords

EGFR
GPNMB
Head and neck squamous cell carcinoma
Immunohistochemistry
VEGF

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van Schaik, J. E., van der Vegt, B., Slagter-Menkema, L., Hanemaaijer, S. H., Halmos, G. B., Witjes, M. J. H., van der Laan, B. F. A. M., Fehrmann, R. S. N., Oosting, S. F., & Plaat, B. E. C. (2024). Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases. American Journal of Otolaryngology - Head and Neck Medicine and Surgery, 45(4), Article 104298. https://doi.org/10.1016/j.amjoto.2024.104298

@article{22bcde4a98474a8aad3e2033d2a5de68,

title = "Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases",

abstract = "Purpose: To investigate glycoprotein nonmetastatic melanoma protein B (GPNMB) and vascular endothelial growth factor (VEGF) as potential fluorescent imaging markers by comparing their protein expression to epidermal growth factor receptor (EGFR).Materials and methods: Thirty-eight paired samples of untreated head and neck squamous cell carcinoma (HNSCC) primary tumours (PT) and corresponding synchronous lymph node metastases (LNM) were selected. After immunohistochemical staining, expression was assessed and compared by the percentage of positive tumour cells. Data were analysed using the Mann-Whitney test, effect sizes (ESr) and Spearman's correlation coefficient (r).Results: GPNMB expression was observed in 100 % of PT, and median 80 % (range 5–100 %) of tumour cells, VEGF in 92 % and 60 % (0–100 %), EGFR in 87 % and 60 % (0–100 %) respectively. In corresponding LNM, GPNMB expression was observed in 100 % of LNM and median 90 % (20–100 %) of tumour cells, VEGF in 87 % and 65 % (0–100 %), and EGFR in 84 % and 35 % (0–100 %). A positive correlation was found between expression in PT and LNM for GPNMB (r = 0.548) and EGFR (r = 0.618) (p < 0.001), but not for VEGF (r = −0.020; p = 0.905). GPNMB expression was present in a higher percentage of tumour cells compared to EGFR in PT (p = 0.015, ESr = −0.320) and in LNM (p < 0.001, ESr = −0.478), while VEGF was not (p = 1.00, ESr = −0.109 and − 0.152, respectively).Conclusion: GPNMB expression is higher than EGFR in untreated HNSCC PT and corresponding LNM, while VEGF expression is comparable to EGFR. GPNMB is a promising target for fluorescent imaging in HNSCC.",

keywords = "EGFR, GPNMB, Head and neck squamous cell carcinoma, Immunohistochemistry, VEGF",

author = "{van Schaik}, {Jeroen E.} and {van der Vegt}, Bert and Lorian Slagter-Menkema and Hanemaaijer, {Saskia H.} and Halmos, {Gyorgi B.} and Witjes, {Max J.H.} and {van der Laan}, {Bernard F.A.M.} and Fehrmann, {Rudolf S.N.} and Oosting, {Sjoukje F.} and Plaat, {Boudewijn E.C.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = jul,

day = "1",

doi = "10.1016/j.amjoto.2024.104298",

language = "English",

volume = "45",

journal = "American Journal of Otolaryngology - Head and Neck Medicine and Surgery",

issn = "0196-0709",

publisher = "W B SAUNDERS CO-ELSEVIER INC",

number = "4",

}

van Schaik, JE , van der Vegt, B, Slagter-Menkema, L, Hanemaaijer, SH , Halmos, GB , Witjes, MJH , van der Laan, BFAM , Fehrmann, RSN , Oosting, SF & Plaat, BEC 2024, 'Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases', American Journal of Otolaryngology - Head and Neck Medicine and Surgery, vol. 45, no. 4, 104298. https://doi.org/10.1016/j.amjoto.2024.104298

Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases. / van Schaik, Jeroen E.; van der Vegt, Bert; Slagter-Menkema, Lorian et al.
In: American Journal of Otolaryngology - Head and Neck Medicine and Surgery, Vol. 45, No. 4, 104298, 01.07.2024.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases

AU - van Schaik, Jeroen E.

AU - van der Vegt, Bert

AU - Slagter-Menkema, Lorian

AU - Hanemaaijer, Saskia H.

AU - Halmos, Gyorgi B.

AU - Witjes, Max J.H.

AU - van der Laan, Bernard F.A.M.

AU - Fehrmann, Rudolf S.N.

AU - Oosting, Sjoukje F.

AU - Plaat, Boudewijn E.C.

PY - 2024/7/1

Y1 - 2024/7/1

N2 - Purpose: To investigate glycoprotein nonmetastatic melanoma protein B (GPNMB) and vascular endothelial growth factor (VEGF) as potential fluorescent imaging markers by comparing their protein expression to epidermal growth factor receptor (EGFR).Materials and methods: Thirty-eight paired samples of untreated head and neck squamous cell carcinoma (HNSCC) primary tumours (PT) and corresponding synchronous lymph node metastases (LNM) were selected. After immunohistochemical staining, expression was assessed and compared by the percentage of positive tumour cells. Data were analysed using the Mann-Whitney test, effect sizes (ESr) and Spearman's correlation coefficient (r).Results: GPNMB expression was observed in 100 % of PT, and median 80 % (range 5–100 %) of tumour cells, VEGF in 92 % and 60 % (0–100 %), EGFR in 87 % and 60 % (0–100 %) respectively. In corresponding LNM, GPNMB expression was observed in 100 % of LNM and median 90 % (20–100 %) of tumour cells, VEGF in 87 % and 65 % (0–100 %), and EGFR in 84 % and 35 % (0–100 %). A positive correlation was found between expression in PT and LNM for GPNMB (r = 0.548) and EGFR (r = 0.618) (p < 0.001), but not for VEGF (r = −0.020; p = 0.905). GPNMB expression was present in a higher percentage of tumour cells compared to EGFR in PT (p = 0.015, ESr = −0.320) and in LNM (p < 0.001, ESr = −0.478), while VEGF was not (p = 1.00, ESr = −0.109 and − 0.152, respectively).Conclusion: GPNMB expression is higher than EGFR in untreated HNSCC PT and corresponding LNM, while VEGF expression is comparable to EGFR. GPNMB is a promising target for fluorescent imaging in HNSCC.

AB - Purpose: To investigate glycoprotein nonmetastatic melanoma protein B (GPNMB) and vascular endothelial growth factor (VEGF) as potential fluorescent imaging markers by comparing their protein expression to epidermal growth factor receptor (EGFR).Materials and methods: Thirty-eight paired samples of untreated head and neck squamous cell carcinoma (HNSCC) primary tumours (PT) and corresponding synchronous lymph node metastases (LNM) were selected. After immunohistochemical staining, expression was assessed and compared by the percentage of positive tumour cells. Data were analysed using the Mann-Whitney test, effect sizes (ESr) and Spearman's correlation coefficient (r).Results: GPNMB expression was observed in 100 % of PT, and median 80 % (range 5–100 %) of tumour cells, VEGF in 92 % and 60 % (0–100 %), EGFR in 87 % and 60 % (0–100 %) respectively. In corresponding LNM, GPNMB expression was observed in 100 % of LNM and median 90 % (20–100 %) of tumour cells, VEGF in 87 % and 65 % (0–100 %), and EGFR in 84 % and 35 % (0–100 %). A positive correlation was found between expression in PT and LNM for GPNMB (r = 0.548) and EGFR (r = 0.618) (p < 0.001), but not for VEGF (r = −0.020; p = 0.905). GPNMB expression was present in a higher percentage of tumour cells compared to EGFR in PT (p = 0.015, ESr = −0.320) and in LNM (p < 0.001, ESr = −0.478), while VEGF was not (p = 1.00, ESr = −0.109 and − 0.152, respectively).Conclusion: GPNMB expression is higher than EGFR in untreated HNSCC PT and corresponding LNM, while VEGF expression is comparable to EGFR. GPNMB is a promising target for fluorescent imaging in HNSCC.

KW - EGFR

KW - GPNMB

KW - Head and neck squamous cell carcinoma

KW - Immunohistochemistry

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=85190523404&partnerID=8YFLogxK

U2 - 10.1016/j.amjoto.2024.104298

DO - 10.1016/j.amjoto.2024.104298

M3 - Article

AN - SCOPUS:85190523404

SN - 0196-0709

VL - 45

JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery

JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery

IS - 4

M1 - 104298

ER -

Potential imaging targets in primary head and neck squamous cell carcinoma and lymph node metastases

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