TY - JOUR
T1 - Pre-B cells in rat bone marrow
T2 - Identification, surface markers and isolation
AU - Opstelten , D
AU - Deenen, GJ
AU - Rozing , J
AU - Hunt, SV
PY - 1985
Y1 - 1985
N2 - In adult rats, as in other species, bone marrow has the highest potential for B-lymphocyte genesis as determined by a long-term repopulation assay for B-lymphocyte stem cells (1). These cells carry the Thy-1 and W3/13 antigens (2, 3). Pre-B cells characterized by the presence of µ heavy chains in the cytoplasm (cµ) but not on the cell surface (sµ) have been described in mice (4), men (5) and rabbits (6) but not in rats, and appear to be the immediate precursors of B-lymphocytes (7–10). The relation between pre-B cells and B-lymphocyte stem cells is not clear, however. Therefore, the aims of the present study were to identify cµ+sµ- pre-B cells in rat bone marrow and to find surface markers that would permit isolation of viable pre-B cells to subsequently assay their functional potential in vivo.
AB - In adult rats, as in other species, bone marrow has the highest potential for B-lymphocyte genesis as determined by a long-term repopulation assay for B-lymphocyte stem cells (1). These cells carry the Thy-1 and W3/13 antigens (2, 3). Pre-B cells characterized by the presence of µ heavy chains in the cytoplasm (cµ) but not on the cell surface (sµ) have been described in mice (4), men (5) and rabbits (6) but not in rats, and appear to be the immediate precursors of B-lymphocytes (7–10). The relation between pre-B cells and B-lymphocyte stem cells is not clear, however. Therefore, the aims of the present study were to identify cµ+sµ- pre-B cells in rat bone marrow and to find surface markers that would permit isolation of viable pre-B cells to subsequently assay their functional potential in vivo.
U2 - 10.1007/978-1-4613-2463-8_2
DO - 10.1007/978-1-4613-2463-8_2
M3 - Review article
SN - 0065-2598
VL - 186
SP - 9
EP - 16
JO - Advances in experimental medicine and biology
JF - Advances in experimental medicine and biology
ER -