Prebiotic oligosaccharides and the enterohepatic circulation of bile salts in rats

Hester van Meer, Gunther Boehm, Frans Stellaard, Aldwin Vriesema, Jan Knol, Rick Havinga, Pieter J. Sauer, Henkjan J. Verkade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Human milk contains prebiotic oligosaccharides, which stimulate the growth of intestinal bifidobacteria and lactobacilli. It is unclear whether the prebiotic capacity of human milk contributes to the larger bile salt pool size and the more efficient fat absorption in infants fed human milk compared with formula. We determined the effect of prebiotic oligosaccharides on bile salt metabolism in rats. Rats were fed a control diet or an isocaloric diet containing a mixture of galactooligosaccharides ( GOS), long-chain fructooligosaccharides (lcFOS), and acidified oligosaccharides (AOS) for 3 wk. We determined synthesis rate, pool size, and fractional turnover rate (FTR) of the primary bile salt cholate by using stable isotope dilution methodology. We quantified bile flow and biliary bile salt secretion rates through bile cannulation. Prebiotic intervention resulted in significant changes in fecal and colonic flora: the proportion of lactobacilli increased 344% ( P <0.01) in colon content and 139% ( P <0.01) in feces compared with the control group. The number of bifidobacteria also increased 366% ( P <0.01) in colon content and 282% in feces after the prebiotic treatment. Furthermore, pH in both colon and feces decreased significantly with 1.0 and 0.5 pH point, respectively. However, despite this alteration of intestinal bacterial flora, no significant effect on relevant parameters of bile salt metabolism and cholate kinetics was found. The present data in rats do not support the hypothesis that prebiotics naturally present in human milk contribute to a larger bile salt pool size or altered bile salt pool kinetics.

Original languageEnglish
Pages (from-to)G540-G547
Number of pages8
JournalAmerican Journal of Physiology-Gastrointestinal and Liver Physiology
Volume294
Issue number2
DOIs
Publication statusPublished - Feb-2008

Keywords

  • bile salt kinetics
  • infant nutrition
  • cholate
  • HUMAN-MILK OLIGOSACCHARIDES
  • INFANT FORMULA
  • FRUCTO-OLIGOSACCHARIDES
  • GALACTO-OLIGOSACCHARIDE
  • SERUM-CHOLESTEROL
  • LIPID-METABOLISM
  • PRETERM INFANTS
  • ACID KINETICS
  • X-RECEPTOR
  • MICROFLORA

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