Predicting sample success for large‐scale ancient DNA studies on marine mammals

Xenia Keighley*, Maiken Hemme Bro‐Jørgensen, Hans Ahlgren, Paul Szpak, Marta Maria Ciucani, Fátima Sánchez Barreiro, Lesley Howse, Anne Birgitte Gotfredsen, Aikaterini Glykou, Peter Jordan, Kerstin Lidén, Morten Tange Olsen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
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In recent years, non‐human ancient DNA studies have begun to focus on larger sample sizes and whole genomes, offering the potential to reveal exciting and hitherto unknown answers to ongoing biological and archaeological questions. However, one major limitation to the feasibility of such studies is the substantial financial and time investments still required during sample screening, due to uncertainty regarding successful sample selection. This study investigates the effect of a wide range of sample properties including latitude, sample age, skeletal element, collagen preservation, and context on endogenous content and DNA damage profiles for 317 ancient and historic pinniped samples collected from across the North Atlantic. Using generalised linear and mixed‐effect models, we found that a range of factors affected DNA preservation within each of the species under consideration. The most important findings were that endogenous content varied significantly according to context, the type of skeletal element, the collagen content and collection year. There also appears to be an effect of the sample’s geographic origin, with samples from the Arctic generally showing higher endogenous content and lower damage rates. Both latitude and sample age were found to have significant relationships with damage levels, but only for walrus samples. Sex, ontogenetic age and extraction material preparation were not found to have any significant relationship with DNA preservation. Overall, the skeletal element and sample context were found to be the most influential factors and should therefore be considered when selecting samples for large‐scale ancient genome studies.
Original languageEnglish
Pages (from-to)1149-1166
Number of pages18
JournalMolecular Ecology Resources
Issue number4
Publication statusPublished - May-2021

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