Preliminary X-ray Study of Naproxen Esterase from Bacillus subtilis

Jan van der Laan; A.V. Teplyakov; A.A. Lammers; B.W. Dijkstra

doi:10.1006/jmbi.1993.1184

Preliminary X-ray Study of Naproxen Esterase from Bacillus subtilis

Jan van der Laan, A.V. Teplyakov, A.A. Lammers, B.W. Dijkstra

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

5 Citations (Scopus)

Abstract

Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4°C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3121 or P3221 with a = b = 47.59 Å and c = 212.91 Å. The asymmetric part of the unit cell contains one protein molecule with Mr = 33,771. The crystals diffract to at least 3.0 Å resolution and are suitable for an X-ray structure analysis.

Original language	English
Pages (from-to)	681-683
Number of pages	3
Journal	Journal of Molecular Biology
Volume	230
Issue number	2
DOIs	https://doi.org/10.1006/jmbi.1993.1184
Publication status	Published - 20-Mar-1993

Keywords

CRYSTALLIZATION
ESTERASE
NAPROXEN
ENANTIOMERIC RESOLUTION
NONSTEROIDAL ANTIINFLAMMATORY AGENTS
ASYMMETRIC HYDROLYSIS
RESOLUTION
ACID
PHARMACOKINETICS
STEREOCHEMISTRY
ENZYMES
GENES
DRUGS

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@article{a65f8733580c4de9827c268210b0f4ef,

title = "Preliminary X-ray Study of Naproxen Esterase from Bacillus subtilis",

abstract = "Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4°C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3121 or P3221 with a = b = 47.59 {\AA} and c = 212.91 {\AA}. The asymmetric part of the unit cell contains one protein molecule with Mr = 33,771. The crystals diffract to at least 3.0 {\AA} resolution and are suitable for an X-ray structure analysis.",

keywords = "CRYSTALLIZATION, ESTERASE, NAPROXEN, ENANTIOMERIC RESOLUTION, NONSTEROIDAL ANTIINFLAMMATORY AGENTS, ASYMMETRIC HYDROLYSIS, RESOLUTION, ACID, PHARMACOKINETICS, STEREOCHEMISTRY, ENZYMES, GENES, DRUGS",

author = "{van der Laan}, Jan and A.V. Teplyakov and A.A. Lammers and B.W. Dijkstra",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1993",

month = mar,

day = "20",

doi = "10.1006/jmbi.1993.1184",

language = "English",

volume = "230",

pages = "681--683",

journal = "Journal of Molecular Biology",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Preliminary X-ray Study of Naproxen Esterase from Bacillus subtilis

AU - van der Laan, Jan

AU - Teplyakov, A.V.

AU - Lammers, A.A.

AU - Dijkstra, B.W.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1993/3/20

Y1 - 1993/3/20

N2 - Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4°C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3121 or P3221 with a = b = 47.59 Å and c = 212.91 Å. The asymmetric part of the unit cell contains one protein molecule with Mr = 33,771. The crystals diffract to at least 3.0 Å resolution and are suitable for an X-ray structure analysis.

AB - Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4°C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3121 or P3221 with a = b = 47.59 Å and c = 212.91 Å. The asymmetric part of the unit cell contains one protein molecule with Mr = 33,771. The crystals diffract to at least 3.0 Å resolution and are suitable for an X-ray structure analysis.

KW - CRYSTALLIZATION

KW - ESTERASE

KW - NAPROXEN

KW - ENANTIOMERIC RESOLUTION

KW - NONSTEROIDAL ANTIINFLAMMATORY AGENTS

KW - ASYMMETRIC HYDROLYSIS

KW - RESOLUTION

KW - ACID

KW - PHARMACOKINETICS

KW - STEREOCHEMISTRY

KW - ENZYMES

KW - GENES

KW - DRUGS

U2 - 10.1006/jmbi.1993.1184

DO - 10.1006/jmbi.1993.1184

M3 - Comment/Letter to the editor

VL - 230

SP - 681

EP - 683

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 2

ER -

Preliminary X-ray Study of Naproxen Esterase from Bacillus subtilis

Abstract

Keywords

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