Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies

Inge A. M. de Graaf*, Peter Olinga, Marina H. de Jager, M.T. Merema, Ruben de Kanter, Esther G. van de Kerkhof, Geny M. M. Groothuis

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

220 Citations (Scopus)

Abstract

Precision-cut tissue slices (PCTS) are viable ex vivo explants of tissue with a reproducible, well defined thickness. They represent a mini-model of the organ under study and contain all cells of the tissue in their natural environment, leaving intercellular and cell-matrix interactions intact, and are therefore highly appropriate for studying multicellular processes. PCTS are mainly used to study the metabolism and toxicity of xenobiotics, but they are suitable for many other purposes. Here we describe the protocols to prepare and incubate rat and human liver and intestinal slices. Slices are prepared from fresh liver by making a cylindrical core using a drill with a hollow bit, from which slices are cut with a specially designed tissue slicer. Intestinal tissue is embedded in cylinders of agarose before slicing. Slices remain viable for 24 h (intestine) and up to 96 h (liver) when incubated in 6- or 12-well plates under 95% O(2)/5% CO(2) atmosphere.

Original languageEnglish
Pages (from-to)1540-1551
Number of pages12
JournalNature protocols
Volume5
Issue number9
DOIs
Publication statusPublished - Sep-2010

Keywords

  • IN-VITRO MODEL
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • DYNAMIC ORGAN-CULTURE
  • ANTI-FIBROTIC DRUGS
  • RAT-LIVER
  • TISSUE-SLICES
  • ISCHEMIA-REPERFUSION
  • ISOLATED HEPATOCYTES
  • MANNOSE 6-PHOSPHATE
  • COLLAGEN SANDWICH

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