@inbook{09b8116ab38b4edfa0669663cc83088c,
title = "Preparation of Cytolysin A (ClyA) Nanopores",
abstract = "The ionic currents passing through nanopores can be used to sequence DNA and identify molecules at the single-molecule level. Recently, researchers have started using nanopores for the detection and analysis of proteins, providing a new platform for single-molecule enzymology studies and more efficient biomolecular sensing applications. For this approach, the homo-oligomeric Cytolysin A (ClyA) nanopore has been demonstrated as a powerful tool. Here, we describe a simple protocol allowing the production of ClyA nanopores. Monomers of ClyA are expressed in Escherichia coli and oligomerized in the presence of detergent. Subsequently, different oligomer variants are electrophoretically resolved and stored in a gel matrix for long-term use.",
keywords = "Nanopores, Cytolysin A, ClyA, protein purification, protein assembly, pore-forming toxin, membrane protein, single-molecule, protein trapping, electrophysiology",
author = "Galenkamp, {Nicole St{\'e}phanie} and {Van Meervelt}, Veerle and Mutter, {Natalie Lisa} and {van der Heide}, {Nieck Jordy} and Carsten Wloka and Giovanni Maglia",
year = "2021",
doi = "10.1007/978-1-0716-0806-7_2",
language = "English",
isbn = "978-1-0716-0805-0",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "11--18",
editor = "{Fahie }, M.A.",
booktitle = "Nanopore Technology",
}