Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis

Corina C.P.M. Bens; Andreas Voss; Corné H.W. Klaassen

doi:10.1128/JCM.44.5.1875-1876.2006

Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis

Corina C.P.M. Bens, Andreas Voss, Corné H.W. Klaassen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

121 Citations (Scopus)

12 Downloads (Pure)

Abstract

Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.

Original language	English
Pages (from-to)	1875-1876
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1128/JCM.44.5.1875-1876.2006
Publication status	Published - May-2006
Externally published	Yes

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10.1128/JCM.44.5.1875-1876.2006

Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis AnalysisFinal publisher's version, 105 KBLicence: Taverne

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Bens, C. C. P. M., Voss, A., & Klaassen, C. H. W. (2006). Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. Journal of Clinical Microbiology, 44(5), 1875-1876. https://doi.org/10.1128/JCM.44.5.1875-1876.2006

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title = "Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis",

abstract = "Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.",

author = "Bens, {Corina C.P.M.} and Andreas Voss and Klaassen, {Corn{\'e} H.W.}",

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Bens, CCPM, Voss, A & Klaassen, CHW 2006, 'Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis', Journal of Clinical Microbiology, vol. 44, no. 5, pp. 1875-1876. https://doi.org/10.1128/JCM.44.5.1875-1876.2006

Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. / Bens, Corina C.P.M.; Voss, Andreas; Klaassen, Corné H.W.
In: Journal of Clinical Microbiology, Vol. 44, No. 5, 05.2006, p. 1875-1876.

Research output: Contribution to journal › Article › Academic › peer-review

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