Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM

LI Los; RJ van der Worp; MJA van Luyn; JMM Hooymans

doi:10.1369/jhc.4A6267.2004

Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM

LI Los^*
, RJ van der Worp
, MJA van Luyn
, JMM Hooymans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.

Original language	English
Pages (from-to)	789-795
Number of pages	7
Journal	Journal of Histochemistry & Cytochemistry
Volume	52
Issue number	6
DOIs	https://doi.org/10.1369/jhc.4A6267.2004
Publication status	Published - Jun-2004

Keywords

collagen IV
ciliary zonules
immunohistochemistry
CHONDROITIN SULFATE PROTEOGLYCANS
EXTRACELLULAR-MATRIX
RAT EYE
MICROFIBRILS
COMPONENTS
EPITHELIUM
EXPRESSION
BODY

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@article{4a7ee751853749a580f2a7affb27748a,

title = "Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM",

abstract = "The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.",

keywords = "collagen IV, ciliary zonules, immunohistochemistry, CHONDROITIN SULFATE PROTEOGLYCANS, EXTRACELLULAR-MATRIX, RAT EYE, MICROFIBRILS, COMPONENTS, EPITHELIUM, EXPRESSION, BODY",

author = "LI Los and \{van der Worp\}, RJ and \{van Luyn\}, MJA and JMM Hooymans",

year = "2004",

month = jun,

doi = "10.1369/jhc.4A6267.2004",

language = "English",

volume = "52",

pages = "789--795",

journal = "Journal of Histochemistry \& Cytochemistry",

issn = "0022-1554",

number = "6",

}

TY - JOUR

T1 - Presence of collagen IV in the ciliary zonules of the human eye

T2 - An immunohistochemical study by LM and TEM

AU - Los, LI

AU - van der Worp, RJ

AU - van Luyn, MJA

AU - Hooymans, JMM

PY - 2004/6

Y1 - 2004/6

N2 - The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.

AB - The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.

KW - collagen IV

KW - ciliary zonules

KW - immunohistochemistry

KW - CHONDROITIN SULFATE PROTEOGLYCANS

KW - EXTRACELLULAR-MATRIX

KW - RAT EYE

KW - MICROFIBRILS

KW - COMPONENTS

KW - EPITHELIUM

KW - EXPRESSION

KW - BODY

U2 - 10.1369/jhc.4A6267.2004

DO - 10.1369/jhc.4A6267.2004

M3 - Article

SN - 0022-1554

VL - 52

SP - 789

EP - 795

JO - Journal of Histochemistry & Cytochemistry

JF - Journal of Histochemistry & Cytochemistry

IS - 6

ER -

Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM

Abstract

Keywords

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