Abstract
In this chapter we outline the procedure for designing oligonucleotide primers for the direct-sequencing of in vitro amplified animal mitochondrial DNA (mtDNA). Although this chapter deals with animal mtDNA in particular, the approach is of course applicable to any gene, nuclear or mitochondrial. There are four main ‘steps’:
identification of a suitable target region;
design of a preliminary set of oligonucleotide primers;
confirmation of sequence identity;
optimization of amplification protocol.
identification of a suitable target region;
design of a preliminary set of oligonucleotide primers;
confirmation of sequence identity;
optimization of amplification protocol.
| Original language | English |
|---|---|
| Title of host publication | Molecular tools for screening biodiversity |
| Subtitle of host publication | Plants and animals |
| Editors | Angela Karp, Peter G. Isaac, David S. Ingram |
| Publisher | Chapman & Hall |
| Pages | 249-255 |
| Number of pages | 7 |
| ISBN (Electronic) | 978-94-009-0019-6 |
| ISBN (Print) | 978-94-010-6496-5 |
| DOIs | |
| Publication status | Published - 1998 |