Progenitor cell niche senescence reflects pathology of the parotid salivary gland in primary Sjogren's syndrome

Xiaoyan Wang; Hendrika Bootsma; Janneke Terpstra; Arjan Vissink; Bert van der Vegt; Fred K L Spijkervet; Frans G M Kroese; Sarah Pringle

doi:10.1093/rheumatology/keaa012

Progenitor cell niche senescence reflects pathology of the parotid salivary gland in primary Sjogren's syndrome

Xiaoyan Wang^*, Hendrika Bootsma, Janneke Terpstra, Arjan Vissink, Bert van der Vegt, Fred K L Spijkervet, Frans G M Kroese, Sarah Pringle

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Objective. Salivary gland (SG) progenitor cells (SGPCs) maintain SG homeostasis. We have previously shown that in primary Sjogren's syndrome (pSS), SGPCs are likely to be senescent, and may underpin SG dysfunction. This study assessed the extent of senescence of cells in a SGPC niche in pSS patients' SGs, and its correlation with functional and clinical parameters.

Methods. The expression of p16 and p21 as markers of senescence in both total SG epithelium and a SGPC niche (basal striated duct cells, BSD) was examined in SGs of pSS (n = 35) , incomplete pSS (n = 1 3) (patients with some signs of pSS, but not fulfilling all classification criteria) and non-SS sicca control (n = 21) patients. This was correlated with functional and clinical parameters.

Results. pSS patient SGs contained significantly more p16(+) cells both in the epithelium in general (P

Conclusion. These findings suggest SGPC senescence may be an early feature of primary Sjogren's syndrome and may contribute to defective SG function in pSS but not to systemic disease activity.

Original language	English
Pages (from-to)	3003-3013
Number of pages	11
Journal	Rheumatology
Volume	59
Issue number	10
Early online date	2020
DOIs	https://doi.org/10.1093/rheumatology/keaa012
Publication status	Published - 1-Oct-2020

Keywords

primary Sjogren's syndrome
salivary gland progenitor cells
senescence
salivary gland
p16
CLASSIFICATION CRITERIA
DIAGNOSIS
PATHOGENESIS
PROGRESSION
EXPRESSION
US

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@article{8bfb387dfe61405d863029310d4387a0,

title = "Progenitor cell niche senescence reflects pathology of the parotid salivary gland in primary Sjogren's syndrome",

abstract = "Objective. Salivary gland (SG) progenitor cells (SGPCs) maintain SG homeostasis. We have previously shown that in primary Sjogren's syndrome (pSS), SGPCs are likely to be senescent, and may underpin SG dysfunction. This study assessed the extent of senescence of cells in a SGPC niche in pSS patients' SGs, and its correlation with functional and clinical parameters.Methods. The expression of p16 and p21 as markers of senescence in both total SG epithelium and a SGPC niche (basal striated duct cells, BSD) was examined in SGs of pSS (n = 35) , incomplete pSS (n = 1 3) (patients with some signs of pSS, but not fulfilling all classification criteria) and non-SS sicca control (n = 21) patients. This was correlated with functional and clinical parameters.Results. pSS patient SGs contained significantly more p16(+) cells both in the epithelium in general (PConclusion. These findings suggest SGPC senescence may be an early feature of primary Sjogren's syndrome and may contribute to defective SG function in pSS but not to systemic disease activity.",

keywords = "primary Sjogren's syndrome, salivary gland progenitor cells, senescence, salivary gland, p16, CLASSIFICATION CRITERIA, DIAGNOSIS, PATHOGENESIS, PROGRESSION, EXPRESSION, US",

author = "Xiaoyan Wang and Hendrika Bootsma and Janneke Terpstra and Arjan Vissink and {van der Vegt}, Bert and Spijkervet, {Fred K L} and Kroese, {Frans G M} and Sarah Pringle",

note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology.",

year = "2020",

month = oct,

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doi = "10.1093/rheumatology/keaa012",

language = "English",

volume = "59",

pages = "3003--3013",

journal = "Rheumatology",

issn = "1462-0324",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Progenitor cell niche senescence reflects pathology of the parotid salivary gland in primary Sjogren's syndrome

AU - Wang, Xiaoyan

AU - Bootsma, Hendrika

AU - Terpstra, Janneke

AU - Vissink, Arjan

AU - van der Vegt, Bert

AU - Spijkervet, Fred K L

AU - Kroese, Frans G M

AU - Pringle, Sarah

N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Objective. Salivary gland (SG) progenitor cells (SGPCs) maintain SG homeostasis. We have previously shown that in primary Sjogren's syndrome (pSS), SGPCs are likely to be senescent, and may underpin SG dysfunction. This study assessed the extent of senescence of cells in a SGPC niche in pSS patients' SGs, and its correlation with functional and clinical parameters.Methods. The expression of p16 and p21 as markers of senescence in both total SG epithelium and a SGPC niche (basal striated duct cells, BSD) was examined in SGs of pSS (n = 35) , incomplete pSS (n = 1 3) (patients with some signs of pSS, but not fulfilling all classification criteria) and non-SS sicca control (n = 21) patients. This was correlated with functional and clinical parameters.Results. pSS patient SGs contained significantly more p16(+) cells both in the epithelium in general (PConclusion. These findings suggest SGPC senescence may be an early feature of primary Sjogren's syndrome and may contribute to defective SG function in pSS but not to systemic disease activity.

AB - Objective. Salivary gland (SG) progenitor cells (SGPCs) maintain SG homeostasis. We have previously shown that in primary Sjogren's syndrome (pSS), SGPCs are likely to be senescent, and may underpin SG dysfunction. This study assessed the extent of senescence of cells in a SGPC niche in pSS patients' SGs, and its correlation with functional and clinical parameters.Methods. The expression of p16 and p21 as markers of senescence in both total SG epithelium and a SGPC niche (basal striated duct cells, BSD) was examined in SGs of pSS (n = 35) , incomplete pSS (n = 1 3) (patients with some signs of pSS, but not fulfilling all classification criteria) and non-SS sicca control (n = 21) patients. This was correlated with functional and clinical parameters.Results. pSS patient SGs contained significantly more p16(+) cells both in the epithelium in general (PConclusion. These findings suggest SGPC senescence may be an early feature of primary Sjogren's syndrome and may contribute to defective SG function in pSS but not to systemic disease activity.

KW - primary Sjogren's syndrome

KW - salivary gland progenitor cells

KW - senescence

KW - salivary gland

KW - p16

KW - CLASSIFICATION CRITERIA

KW - DIAGNOSIS

KW - PATHOGENESIS

KW - PROGRESSION

KW - EXPRESSION

KW - US

U2 - 10.1093/rheumatology/keaa012

DO - 10.1093/rheumatology/keaa012

M3 - Article

C2 - 32159757

SN - 1462-0324

VL - 59

SP - 3003

EP - 3013

JO - Rheumatology

JF - Rheumatology

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