Prolonged platelet preservation by transient metabolic suppression

B A Badlou, M J W Ijseldijk, W M Smid, J W N Akkerman

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    Abstract

    BACKGROUND: In this study whether metabolic suppression can be used to preserve platelet (PLT) function during prolonged storage was investigated.

    STUDY DESIGN AND METHODS: Washed human PLTs were incubated without glucose and with antimycin A to block energy generation. Metabolic suppressed PLTs (MSPs) were stored for 72 hours at different temperatures to find the optimal storage temperature. Controls were incubated with 5 mmol per L glucose and stored at 22 and 4 degrees C.

    RESULTS: Following metabolic recovery with glucose, MSPs stored at 37, 22, and 4 degrees C showed an increase in basal P-selectin expression (PSE) reaching greater than 40 percent after about 2, 20, and 48 hours; a decrease in thrombin receptor-activating peptide SFLLRN (TRAP)-induced PSE inversely related to the increase in basal PSE; and a decrease in TRAP-induced aggregation reaching less than 30 percent after about 4, 24, and more than 72 hours. When compared with control suspensions, MSPs stored at 4 degrees C better preserved a low basal PSE and in addition showed a better adhesion to surface coated-von Willebrand factor and fibrinogen in a flow chamber.

    CONCLUSION: Metabolic suppression before storage at 4 degrees C contributes to better preservation of PLT function.

    Original languageEnglish
    Pages (from-to)214-22
    Number of pages9
    JournalTransfusion
    Volume45
    Issue number2
    DOIs
    Publication statusPublished - Feb-2005

    Keywords

    • Blood Platelets/metabolism
    • Blood Preservation/methods
    • Cold Temperature
    • Energy Metabolism
    • Fibrinogen/metabolism
    • Glucose/metabolism
    • Humans
    • P-Selectin
    • Platelet Adhesiveness
    • Platelet Aggregation
    • Platelet Glycoprotein GPIb-IX Complex/metabolism
    • Platelet Transfusion
    • von Willebrand Factor/metabolism

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