Abstract
Polyclonal antibodies were raised against LcnC and LcnD proteins of the Lactococcus lactis bacteriocin lactococcin A secretory system to examine their cellular location and interaction. Two major reacting bands were detected by Western immunoblot with the anti-LcnD antibody: one of 52 kDa (LcnD) and another of 45 kDa, called here LcnD*. LcnD* was still detectable after removing the AUG start codon for LcnD. Chemical cross-linking analyses of membrane fractions of L. lactis cells expressing the LcnC/D secretion machinery were performed. Our results indicate that LcnD is present in the secretion machinery complex as a dimer and is able to interact with LcnD* and LcnC. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 259-263 |
Number of pages | 5 |
Journal | FEMS Microbiology Letters |
Volume | 204 |
Issue number | 2 |
DOIs | |
Publication status | Published - 13-Nov-2001 |
Keywords
- lactic acid bacterium
- bacteriocin
- lactococcin A
- protein secretion
- Lactococcus lactis
- LACTIC STREPTOCOCCI
- ESCHERICHIA-COLI
- PLASMID
- BACTERIOCIN
- GENE
- TOPOLOGY
- CVAA