Proteins of the lactococcin A secretion system: lcnD encodes two in-frame proteins

M Varcamonti*, G Nicastro, G Venema, J Kok

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)
267 Downloads (Pure)

Abstract

Polyclonal antibodies were raised against LcnC and LcnD proteins of the Lactococcus lactis bacteriocin lactococcin A secretory system to examine their cellular location and interaction. Two major reacting bands were detected by Western immunoblot with the anti-LcnD antibody: one of 52 kDa (LcnD) and another of 45 kDa, called here LcnD*. LcnD* was still detectable after removing the AUG start codon for LcnD. Chemical cross-linking analyses of membrane fractions of L. lactis cells expressing the LcnC/D secretion machinery were performed. Our results indicate that LcnD is present in the secretion machinery complex as a dimer and is able to interact with LcnD* and LcnC. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalFEMS Microbiology Letters
Volume204
Issue number2
DOIs
Publication statusPublished - 13-Nov-2001

Keywords

  • lactic acid bacterium
  • bacteriocin
  • lactococcin A
  • protein secretion
  • Lactococcus lactis
  • LACTIC STREPTOCOCCI
  • ESCHERICHIA-COLI
  • PLASMID
  • BACTERIOCIN
  • GENE
  • TOPOLOGY
  • CVAA

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