Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells

Tanja Netelenbos; Suzanne Zuijderduijn; Jacob Van Den Born; Floortje L Kessler; Sonja Zweegman; Peter C Huijgens; Angelika M Dräger

doi:10.1189/jlb.72.2.353

Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells

Tanja Netelenbos
, Suzanne Zuijderduijn
, Jacob Van Den Born
, Floortje L Kessler
, Sonja Zweegman
, Peter C Huijgens
, Angelika M Dräger

Research output: Contribution to journal › Article › Academic › peer-review

89 Citations (Scopus)

Abstract

Stromal cell-derived factor-1 (SDF-1) is a chemoattractant involved in hematopoietic progenitor cell (HPC) trafficking to the bone marrow. We studied the role of bone marrow endothelial proteoglycans (PGs) in SDF-1-mediated migration of HPC using a transwell assay. A subclone of progenitor cell line KG-1 (KG-1v) was used, displaying CXCR4-dependent transmigration. Cell surface PGs on bone marrow endothelial cell line 4LHBMEC did not mediate SDF-1-induced transendothelial migration. In contrast, transwell filters precoated with various glycosaminoglycans (GAGs) enhanced migration toward SDF-1. SDF-1-induced migration was reduced by degradation of heparan sulfate in subendothelial matrix produced by 4LHBMEC. The stimulating effect of GAGs was caused by the formation of a stable haptotactic SDF-1 gradient, as SDF-1 bound to immobilized GAGs and triggered migration. Soluble heparan sulfate enhanced SDF-1-induced migration dose-dependently, suggesting that SDF-1-heparan sulfate complexes optimized SDF-1 presentation. In conclusion, we provide evidence that PGs in the subendothelial matrix establish an SDF-1 gradient guiding migrating HPC into the bone marrow.

Original language	English
Pages (from-to)	353-362
Number of pages	10
Journal	Journal of Leukocyte Biology
Volume	72
Issue number	2
DOIs	https://doi.org/10.1189/jlb.72.2.353
Publication status	Published - Aug-2002

Keywords

Bone Marrow/blood supply
Cell Adhesion/drug effects
Cell Movement/drug effects
Cells, Cultured/drug effects
Chemokine CXCL12
Chemokines, CXC/administration & dosage
Dose-Response Relationship, Drug
Endothelium, Vascular/chemistry
Extracellular Matrix/chemistry
Fibronectins/metabolism
HL-60 Cells/drug effects
Hematopoietic Stem Cells/cytology
Heparin/chemistry
Heparitin Sulfate/chemistry
Humans
Macromolecular Substances
Osmolar Concentration
Protein Binding
Proteoglycans/chemistry
Receptors, CXCR4/drug effects
Solubility
Structure-Activity Relationship
Vascular Cell Adhesion Molecule-1/metabolism

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Proteoglycans guide SDF‐1‐induced migration of hematopoietic progenitor cells
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Cite this

@article{5287fee9d9984b6e929e51a8aedef54d,

title = "Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells",

abstract = "Stromal cell-derived factor-1 (SDF-1) is a chemoattractant involved in hematopoietic progenitor cell (HPC) trafficking to the bone marrow. We studied the role of bone marrow endothelial proteoglycans (PGs) in SDF-1-mediated migration of HPC using a transwell assay. A subclone of progenitor cell line KG-1 (KG-1v) was used, displaying CXCR4-dependent transmigration. Cell surface PGs on bone marrow endothelial cell line 4LHBMEC did not mediate SDF-1-induced transendothelial migration. In contrast, transwell filters precoated with various glycosaminoglycans (GAGs) enhanced migration toward SDF-1. SDF-1-induced migration was reduced by degradation of heparan sulfate in subendothelial matrix produced by 4LHBMEC. The stimulating effect of GAGs was caused by the formation of a stable haptotactic SDF-1 gradient, as SDF-1 bound to immobilized GAGs and triggered migration. Soluble heparan sulfate enhanced SDF-1-induced migration dose-dependently, suggesting that SDF-1-heparan sulfate complexes optimized SDF-1 presentation. In conclusion, we provide evidence that PGs in the subendothelial matrix establish an SDF-1 gradient guiding migrating HPC into the bone marrow.",

keywords = "Bone Marrow/blood supply, Cell Adhesion/drug effects, Cell Movement/drug effects, Cells, Cultured/drug effects, Chemokine CXCL12, Chemokines, CXC/administration \& dosage, Dose-Response Relationship, Drug, Endothelium, Vascular/chemistry, Extracellular Matrix/chemistry, Fibronectins/metabolism, HL-60 Cells/drug effects, Hematopoietic Stem Cells/cytology, Heparin/chemistry, Heparitin Sulfate/chemistry, Humans, Macromolecular Substances, Osmolar Concentration, Protein Binding, Proteoglycans/chemistry, Receptors, CXCR4/drug effects, Solubility, Structure-Activity Relationship, Vascular Cell Adhesion Molecule-1/metabolism",

author = "Tanja Netelenbos and Suzanne Zuijderduijn and \{Van Den Born\}, Jacob and Kessler, \{Floortje L\} and Sonja Zweegman and Huijgens, \{Peter C\} and Dr{\"a}ger, \{Angelika M\}",

year = "2002",

month = aug,

doi = "10.1189/jlb.72.2.353",

language = "English",

volume = "72",

pages = "353--362",

journal = "Journal of Leukocyte Biology",

issn = "0741-5400",

publisher = "FEDERATION AMER SOC EXP BIOL",

number = "2",

}

TY - JOUR

T1 - Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells

AU - Netelenbos, Tanja

AU - Zuijderduijn, Suzanne

AU - Van Den Born, Jacob

AU - Kessler, Floortje L

AU - Zweegman, Sonja

AU - Huijgens, Peter C

AU - Dräger, Angelika M

PY - 2002/8

Y1 - 2002/8

N2 - Stromal cell-derived factor-1 (SDF-1) is a chemoattractant involved in hematopoietic progenitor cell (HPC) trafficking to the bone marrow. We studied the role of bone marrow endothelial proteoglycans (PGs) in SDF-1-mediated migration of HPC using a transwell assay. A subclone of progenitor cell line KG-1 (KG-1v) was used, displaying CXCR4-dependent transmigration. Cell surface PGs on bone marrow endothelial cell line 4LHBMEC did not mediate SDF-1-induced transendothelial migration. In contrast, transwell filters precoated with various glycosaminoglycans (GAGs) enhanced migration toward SDF-1. SDF-1-induced migration was reduced by degradation of heparan sulfate in subendothelial matrix produced by 4LHBMEC. The stimulating effect of GAGs was caused by the formation of a stable haptotactic SDF-1 gradient, as SDF-1 bound to immobilized GAGs and triggered migration. Soluble heparan sulfate enhanced SDF-1-induced migration dose-dependently, suggesting that SDF-1-heparan sulfate complexes optimized SDF-1 presentation. In conclusion, we provide evidence that PGs in the subendothelial matrix establish an SDF-1 gradient guiding migrating HPC into the bone marrow.

AB - Stromal cell-derived factor-1 (SDF-1) is a chemoattractant involved in hematopoietic progenitor cell (HPC) trafficking to the bone marrow. We studied the role of bone marrow endothelial proteoglycans (PGs) in SDF-1-mediated migration of HPC using a transwell assay. A subclone of progenitor cell line KG-1 (KG-1v) was used, displaying CXCR4-dependent transmigration. Cell surface PGs on bone marrow endothelial cell line 4LHBMEC did not mediate SDF-1-induced transendothelial migration. In contrast, transwell filters precoated with various glycosaminoglycans (GAGs) enhanced migration toward SDF-1. SDF-1-induced migration was reduced by degradation of heparan sulfate in subendothelial matrix produced by 4LHBMEC. The stimulating effect of GAGs was caused by the formation of a stable haptotactic SDF-1 gradient, as SDF-1 bound to immobilized GAGs and triggered migration. Soluble heparan sulfate enhanced SDF-1-induced migration dose-dependently, suggesting that SDF-1-heparan sulfate complexes optimized SDF-1 presentation. In conclusion, we provide evidence that PGs in the subendothelial matrix establish an SDF-1 gradient guiding migrating HPC into the bone marrow.

KW - Bone Marrow/blood supply

KW - Cell Adhesion/drug effects

KW - Cell Movement/drug effects

KW - Cells, Cultured/drug effects

KW - Chemokine CXCL12

KW - Chemokines, CXC/administration & dosage

KW - Dose-Response Relationship, Drug

KW - Endothelium, Vascular/chemistry

KW - Extracellular Matrix/chemistry

KW - Fibronectins/metabolism

KW - HL-60 Cells/drug effects

KW - Hematopoietic Stem Cells/cytology

KW - Heparin/chemistry

KW - Heparitin Sulfate/chemistry

KW - Humans

KW - Macromolecular Substances

KW - Osmolar Concentration

KW - Protein Binding

KW - Proteoglycans/chemistry

KW - Receptors, CXCR4/drug effects

KW - Solubility

KW - Structure-Activity Relationship

KW - Vascular Cell Adhesion Molecule-1/metabolism

U2 - 10.1189/jlb.72.2.353

DO - 10.1189/jlb.72.2.353

M3 - Article

C2 - 12149427

SN - 0741-5400

VL - 72

SP - 353

EP - 362

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 2

ER -

Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells

Abstract

Keywords

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