TY - JOUR
T1 - Proteomics analysis of Hodgkin lymphoma
T2 - identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes
AU - Ma, Yue
AU - Visser, Lydia
AU - Roelofsen, Johan
AU - de Vries, Marcel
AU - Diepstra, Arjan
AU - van Imhoff, Gustaaf
AU - van der Wal, Tineke
AU - Luinge, Marjan
AU - Alvarez Llamas, Gloria
AU - Vos, Hans
AU - Poppema, Sibrand
AU - Vonk, Roel
AU - van den Berg, Anke
PY - 2008/2/15
Y1 - 2008/2/15
N2 - Hodgkin and Reed-Sternberg (HRS) cells in Hodgkin lymphoma (HL) secrete factors that interact with inflammatory background cells and may serve as biomarkers for disease activity. To detect new proteins related to pathogenesis, we analyzed the secretome of HRS cells. Proteins in cell culture supernatant of 4 HL cell lines were identified using 1 DGE followed by in-gel trypsin digestion and LC-MS/MS. In total, 1290 proteins, including 368 secreted proteins, were identified. Functional grouping of secreted proteins revealed 37 proteins involved in immune response. Sixteen of the 37 proteins (ie, ALCAM, Cathepsin C, Cathepsin S, CD100, CD150, CD26, CD44, CD63, C1371, Fractalkine, IL1R2, 1L25, IP-10, MIF, RANTES, and TARC) were validated in HL cell lines and patient material using immunohistochemistry and/or ELISA. Expression of all 16 proteins was confirmed in HL cell lines, and 15 were also confirmed in HL tissues. Seven proteins (ALCAM, cathepsin S, CD26, CD44, IL1R2, MIF, and TARC) revealed significantly elevated levels in patient plasma compared with healthy controls. Proteomics analyses of HL cell line supernatant allowed detection of new secreted proteins, which may add to our insights in the interaction between HRS cells and infiltrating lymphocytes and in some instances might serve as biomarkers.
AB - Hodgkin and Reed-Sternberg (HRS) cells in Hodgkin lymphoma (HL) secrete factors that interact with inflammatory background cells and may serve as biomarkers for disease activity. To detect new proteins related to pathogenesis, we analyzed the secretome of HRS cells. Proteins in cell culture supernatant of 4 HL cell lines were identified using 1 DGE followed by in-gel trypsin digestion and LC-MS/MS. In total, 1290 proteins, including 368 secreted proteins, were identified. Functional grouping of secreted proteins revealed 37 proteins involved in immune response. Sixteen of the 37 proteins (ie, ALCAM, Cathepsin C, Cathepsin S, CD100, CD150, CD26, CD44, CD63, C1371, Fractalkine, IL1R2, 1L25, IP-10, MIF, RANTES, and TARC) were validated in HL cell lines and patient material using immunohistochemistry and/or ELISA. Expression of all 16 proteins was confirmed in HL cell lines, and 15 were also confirmed in HL tissues. Seven proteins (ALCAM, cathepsin S, CD26, CD44, IL1R2, MIF, and TARC) revealed significantly elevated levels in patient plasma compared with healthy controls. Proteomics analyses of HL cell line supernatant allowed detection of new secreted proteins, which may add to our insights in the interaction between HRS cells and infiltrating lymphocytes and in some instances might serve as biomarkers.
KW - REED-STERNBERG CELLS
KW - DIFFERENTIAL CHEMOKINE EXPRESSION
KW - MIGRATION INHIBITORY FACTOR
KW - AMINO-TERMINAL TRUNCATION
KW - HUMAN B-LYMPHOCYTES
KW - CC CHEMOKINES
KW - RS CELLS
KW - IN-VIVO
KW - DISEASE
KW - RECEPTOR
U2 - 10.1182/blood-2007-09-112128
DO - 10.1182/blood-2007-09-112128
M3 - Article
SN - 0006-4971
VL - 111
SP - 2339
EP - 2346
JO - Blood
JF - Blood
IS - 4
ER -