Protocol to study toxic, immune, and suicidal phenotypes of killer yeast

Rianne C Prins; Sonja Billerbeck

doi:10.1016/j.xpro.2025.103611

Protocol to study toxic, immune, and suicidal phenotypes of killer yeast

Rianne C Prins, Sonja Billerbeck^*

^*Corresponding author for this work

Molecular Microbiology

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Abstract

Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis. For complete details on the use and execution of this protocol, please refer to Prins et al. 1.

Original language	English
Article number	103611
Number of pages	22
Journal	STAR protocols
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2025.103611
Publication status	Published - 21-Mar-2025

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10.1016/j.xpro.2025.103611Licence: CC BY

Protocol to study toxic, immune, and suicidal phenotypes of killer yeastFinal publisher's version, 4.82 MBLicence: CC BY

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abstract = "Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis. For complete details on the use and execution of this protocol, please refer to Prins et al. 1. ",

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N2 - Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis. For complete details on the use and execution of this protocol, please refer to Prins et al. 1.

AB - Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis. For complete details on the use and execution of this protocol, please refer to Prins et al. 1.

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Protocol to study toxic, immune, and suicidal phenotypes of killer yeast

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