Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles

Miroslava Opekarovà; Arnold J.M. Driessen; Wilhelmus Konings

doi:10.1016/0014-5793(87)81462-X

Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles

Miroslava Opekarovà
, Arnold J.M. Driessen
, Wilhelmus Konings

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.

Original language	English
Pages (from-to)	45-48
Number of pages	4
Journal	FEBS Letters
Volume	213
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)81462-X
Publication status	Published - 9-Mar-1987

Keywords

(S. cerevisiae)
Amino acid transport
Leucine
Membrane vesicle

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title = "Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles",

abstract = "Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.",

keywords = "(S. cerevisiae), Amino acid transport, Leucine, Membrane vesicle",

author = "Miroslava Opekarov{\`a} and Driessen, \{Arnold J.M.\} and Wilhelmus Konings",

note = "Relation: http://www.rug.nl/ date\_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

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AU - Opekarovà, Miroslava

AU - Driessen, Arnold J.M.

AU - Konings, Wilhelmus

N1 - Relation: http://www.rug.nl/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1987/3/9

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N2 - Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.

AB - Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.

KW - (S. cerevisiae)

KW - Amino acid transport

KW - Leucine

KW - Membrane vesicle

U2 - 10.1016/0014-5793(87)81462-X

DO - 10.1016/0014-5793(87)81462-X

M3 - Article

SN - 0014-5793

VL - 213

SP - 45

EP - 48

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles

Abstract

Keywords

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