PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

CMH HENSGENS; J VONCK; J VANBEEUMEN; EFJ VANBRUGGEN; TA HANSEN

PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

CMH HENSGENS, J VONCK, J VANBEEUMEN, EFJ VANBRUGGEN, TA HANSEN

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic › peer-review

41 Citations (Scopus)

Abstract

A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.

Original language	English
Pages (from-to)	2859-2863
Number of pages	5
Journal	Journal of Bacteriology
Volume	175
Issue number	10
Publication status	Published - May-1993

Keywords

SULFATE-REDUCING BACTERIA
ZYMOMONAS-MOBILIS
SACCHAROMYCES-CEREVISIAE
ESCHERICHIA-COLI
SP-NOV
GENE
IRON
SEQUENCE
PROTEIN
ETHANOL

Handle.net

Persistent link

Cite this

@article{ef5746a63a7f4233b779e80943a275fb,

title = "PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS",

abstract = "A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.",

keywords = "SULFATE-REDUCING BACTERIA, ZYMOMONAS-MOBILIS, SACCHAROMYCES-CEREVISIAE, ESCHERICHIA-COLI, SP-NOV, GENE, IRON, SEQUENCE, PROTEIN, ETHANOL",

author = "CMH HENSGENS and J VONCK and J VANBEEUMEN and EFJ VANBRUGGEN and TA HANSEN",

year = "1993",

month = may,

language = "English",

volume = "175",

pages = "2859--2863",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

number = "10",

}

TY - JOUR

T1 - PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

AU - HENSGENS, CMH

AU - VONCK, J

AU - VANBEEUMEN, J

AU - VANBRUGGEN, EFJ

AU - HANSEN, TA

PY - 1993/5

Y1 - 1993/5

N2 - A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.

AB - A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.

KW - SULFATE-REDUCING BACTERIA

KW - ZYMOMONAS-MOBILIS

KW - SACCHAROMYCES-CEREVISIAE

KW - ESCHERICHIA-COLI

KW - SP-NOV

KW - GENE

KW - IRON

KW - SEQUENCE

KW - PROTEIN

KW - ETHANOL

M3 - Article

SN - 0021-9193

VL - 175

SP - 2859

EP - 2863

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 10

ER -

PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

Abstract

Keywords

Handle.net

Fingerprint

Cite this