Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid Delta(1) -dehydrogenase from Rhodococcus erythropolis SQ1

A. Rohman, N. van Oosterwijk, B. W. Dijkstra

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Abstract

3-Ketosteroid Delta(1)-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates. The 3-ketosteroid Delta(1)-dehydrogenase from Rhodococcus erythropolis SQ1, a 56 kDa flavoprotein, was crystallized using the sitting-drop vapour-diffusion method at room temperature. The crystals grew in various buffers over a wide pH range (from pH 5.5 to 10.5), but the best crystallization condition consisted of 2%(nu/nu) PEG 400, 0.1 M HEPES pH 7.5, 2.0 M ammonium sulfate. A native crystal diffracted X-rays to 2.0 angstrom resolution. It belonged to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 107.4, b = 131.6, c = 363.2 angstrom, and contained eight molecules in the asymmetric unit. The initial structure of the enzyme was solved using multi-wavelength anomalous dispersion (MAD) data collected from a Pt-derivatized crystal.
Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume68
Issue number5
DOIs
Publication statusPublished - May-2012

Keywords

  • DELTA(1)-DEHYDROGENASE ISOENZYME
  • CHOLESTEROL CATABOLISM
  • NOCARDIA-CORALLINA
  • GENE-CLUSTER
  • STEROIDS
  • 3-KETOSTEROID-DELTA-1-DEHYDROGENASE
  • MECHANISM
  • KSTD

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