Quantification of Biventricular Function and Morphology by Cardiac Magnetic Resonance Imaging in Mice with Pulmonary Artery Banding

Anne-Marie C Koop*, Quint A J Hagdorn, Kees C W A van de Kolk, Annemieke van Oosten, Michel Weij, Herman H W Silljé, Tineke P Willems, Rolf M F Berger

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Right ventricular (RV) function and failure are major determinants of outcome in acquired and congenital heart diseases, including pulmonary hypertension. Assessment of RV function and morphology is complex, partly due to the complex shape of the RV. Currently, cardiac magnetic resonance (CMR) imaging is the golden standard for noninvasive assessment of RV function and morphology. The current protocol describes CMR imaging in a mouse model of RV pressure load induced by pulmonary artery banding (PAB). PAB is performed by placing a 6-0 suture around the pulmonary artery over a 23 G needle. The PAB gradient is determined using echocardiography at 2 and 6 weeks. At 6 weeks, the right and left ventricular morphology and function is assessed by measuring both end-systolic and end-diastolic volumes and mass by ten to eleven cine slices 1 mm thick using a 9.4 T magnetic resonance imaging scanner equipped with a 1,500 mT/m gradient. Representative results show that PAB induces a significant increase in RV pressure load, with significant effects on biventricular morphology and RV function. It is also shown that at 6 weeks of RV pressure load, cardiac output is maintained. Presented here is a reproducible protocol for the quantification of biventricular morphology and function in a mouse model of RV pressure load and may serve as a method for experiments exploring determinants of RV remodeling and dysfunction.

Original languageEnglish
Article number60837
Number of pages10
JournalJournal of visualized experiments : JoVE
Volume2020
Issue number159
DOIs
Publication statusPublished - May-2020

Keywords

  • Neuroscience
  • Issue 159
  • right ventricular pressure load
  • mice model
  • pulmonary artery banding
  • cardiac magnetic resonance
  • ventricular dimensions
  • cardiac remodeling
  • hypertrophy
  • dilatation
  • cardiac function
  • RIGHT-VENTRICULAR HYPERTROPHY
  • PRESSURE-OVERLOAD
  • HEART-FAILURE
  • UP-REGULATION
  • 3-DIMENSIONAL ECHOCARDIOGRAPHY
  • MITOCHONDRIAL DYSFUNCTION
  • EJECTION FRACTION
  • ENERGY-METABOLISM
  • TIME-COURSE
  • HYPOXIA

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