QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

BG WOLTHERS; WDJV KAMERBEEK; CM VANBEUSEKOM; F ELSHOF; AWD BUITENHUIS; EPR BRUNT; JPWF LAKKE

QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

BG WOLTHERS^*, WDJV KAMERBEEK, CM VANBEUSEKOM, F ELSHOF, AWD BUITENHUIS, EPR BRUNT, JPWF LAKKE

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

An HPLC method for the determination of lisuride hydrogen maleate in plasma is described. After addition of ergotamine tartrate as internal standard, plasma is extracted with diethyl ether. Following evaporation of the solvent and redissolving in methanol the extract is injected on a silica HPLC column and lisuride is monitored by fluorescence detection using an excitation wavelength of 322 nm and an emission wavelength of 405 nm. The method is sufficiently accurate and precise with a detection limit of 20 pg/ml lisuride in plasma. The usefulness of the method is demonstrated by measurements of lisuride levels after oral intake of a 0.6 mg dose of the drug by a healthy male volunteer, showing a peak level of 1266 pg/ml, 45 min after intake.

Original language	English
Pages (from-to)	33-38
Number of pages	6
Journal	Journal of chromatography-Biomedical applications
Volume	622
Issue number	1
Publication status	Published - 8-Dec-1993

Keywords

PARKINSONS-DISEASE

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@article{ef2cad1c54584137b4f95547aac7b6f9,

title = "QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION",

abstract = "An HPLC method for the determination of lisuride hydrogen maleate in plasma is described. After addition of ergotamine tartrate as internal standard, plasma is extracted with diethyl ether. Following evaporation of the solvent and redissolving in methanol the extract is injected on a silica HPLC column and lisuride is monitored by fluorescence detection using an excitation wavelength of 322 nm and an emission wavelength of 405 nm. The method is sufficiently accurate and precise with a detection limit of 20 pg/ml lisuride in plasma. The usefulness of the method is demonstrated by measurements of lisuride levels after oral intake of a 0.6 mg dose of the drug by a healthy male volunteer, showing a peak level of 1266 pg/ml, 45 min after intake.",

keywords = "PARKINSONS-DISEASE",

author = "BG WOLTHERS and WDJV KAMERBEEK and CM VANBEUSEKOM and F ELSHOF and AWD BUITENHUIS and EPR BRUNT and JPWF LAKKE",

year = "1993",

month = dec,

day = "8",

language = "English",

volume = "622",

pages = "33--38",

journal = "Journal of chromatography-Biomedical applications",

issn = "0378-4347",

publisher = "ELSEVIER SCIENCE BV",

number = "1",

}

QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION. / WOLTHERS, BG; KAMERBEEK, WDJV; VANBEUSEKOM, CM et al.
In: Journal of chromatography-Biomedical applications, Vol. 622, No. 1, 08.12.1993, p. 33-38.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

AU - WOLTHERS, BG

AU - KAMERBEEK, WDJV

AU - VANBEUSEKOM, CM

AU - ELSHOF, F

AU - BUITENHUIS, AWD

AU - BRUNT, EPR

AU - LAKKE, JPWF

PY - 1993/12/8

Y1 - 1993/12/8

N2 - An HPLC method for the determination of lisuride hydrogen maleate in plasma is described. After addition of ergotamine tartrate as internal standard, plasma is extracted with diethyl ether. Following evaporation of the solvent and redissolving in methanol the extract is injected on a silica HPLC column and lisuride is monitored by fluorescence detection using an excitation wavelength of 322 nm and an emission wavelength of 405 nm. The method is sufficiently accurate and precise with a detection limit of 20 pg/ml lisuride in plasma. The usefulness of the method is demonstrated by measurements of lisuride levels after oral intake of a 0.6 mg dose of the drug by a healthy male volunteer, showing a peak level of 1266 pg/ml, 45 min after intake.

AB - An HPLC method for the determination of lisuride hydrogen maleate in plasma is described. After addition of ergotamine tartrate as internal standard, plasma is extracted with diethyl ether. Following evaporation of the solvent and redissolving in methanol the extract is injected on a silica HPLC column and lisuride is monitored by fluorescence detection using an excitation wavelength of 322 nm and an emission wavelength of 405 nm. The method is sufficiently accurate and precise with a detection limit of 20 pg/ml lisuride in plasma. The usefulness of the method is demonstrated by measurements of lisuride levels after oral intake of a 0.6 mg dose of the drug by a healthy male volunteer, showing a peak level of 1266 pg/ml, 45 min after intake.

KW - PARKINSONS-DISEASE

M3 - Article

SN - 0378-4347

VL - 622

SP - 33

EP - 38

JO - Journal of chromatography-Biomedical applications

JF - Journal of chromatography-Biomedical applications

IS - 1

ER -

QUANTITATIVE-DETERMINATION OF THE DOPAMINE AGONIST LISURIDE IN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

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Keywords

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