Quantitative on-line monitoring of cellular and cerebral energy metabolism

Neither metabolism of the brain in vivo, nor energy use of brain cells in vitro, has been monitored with our on-line system before. In this thesis we have measured glucose and lactate metabolism on-line, both in vitro and in vivo. The on-line system with enzyme- based biosensors allows to measure glucose and lactate concentrations very specifically, whereas the small tubing and very low volumes give us high time resolution and low lag times. We investigated the use of the on-line monitoring system for in vitro and in vivo measurement of brain metabolism, and studied today’s concepts on cellular and cerebral metabolism.