Abstract
An on-line in vitro perfusion technique is described that allows the continuous quantification of cellular glucose metabolism in vitro. Using biosensor technology, we measure glucose and lactate metabolism at a minute-to-minute time resolution for periods up to several days. The application of our perfusion-detection technique for in vitro monitoring is demonstrated in a wide variety of cells, including primary neuronal and astroglia cultures, yeast cells, and human lymphocytes. The method shows that variations in oxygen delivery or exposure to a noncompetitive pseudosubstrate (here 2-deoxyglucose) affects normal glucose metabolism. The innovative advantage of the present system is that, in contrast to other devices including a recently described system, metabolism per cell can be quantified. The potential of in vitro on-line monitoring is discussed for application in studying normal and abnormal metabolism, toxic and nontoxic drug effects, and human tissue biopsies.
Original language | English |
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Pages (from-to) | 5431-5435 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 76 |
Issue number | 18 |
DOIs | |
Publication status | Published - 15-Sep-2004 |
Keywords
- HOLLOW-FIBER BIOREACTOR
- RAT-BRAIN
- ULTRASLOW MICRODIALYSIS
- HYBRIDOMA CELLS
- ENERGY
- ULTRAFILTRATION
- BIOSENSOR
- CULTURE
- SPECTROSCOPY
- COMBINATION