Quantitative on-line monitoring of cellular glucose and lactate metabolism in vitro with slow perfusion

G Leegsma-Vogt, K Venema, N Brouwer, JB Gramsbergen, S Copray, J Korf*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

An on-line in vitro perfusion technique is described that allows the continuous quantification of cellular glucose metabolism in vitro. Using biosensor technology, we measure glucose and lactate metabolism at a minute-to-minute time resolution for periods up to several days. The application of our perfusion-detection technique for in vitro monitoring is demonstrated in a wide variety of cells, including primary neuronal and astroglia cultures, yeast cells, and human lymphocytes. The method shows that variations in oxygen delivery or exposure to a noncompetitive pseudosubstrate (here 2-deoxyglucose) affects normal glucose metabolism. The innovative advantage of the present system is that, in contrast to other devices including a recently described system, metabolism per cell can be quantified. The potential of in vitro on-line monitoring is discussed for application in studying normal and abnormal metabolism, toxic and nontoxic drug effects, and human tissue biopsies.

Original languageEnglish
Pages (from-to)5431-5435
Number of pages5
JournalAnalytical Chemistry
Volume76
Issue number18
DOIs
Publication statusPublished - 15-Sep-2004

Keywords

  • HOLLOW-FIBER BIOREACTOR
  • RAT-BRAIN
  • ULTRASLOW MICRODIALYSIS
  • HYBRIDOMA CELLS
  • ENERGY
  • ULTRAFILTRATION
  • BIOSENSOR
  • CULTURE
  • SPECTROSCOPY
  • COMBINATION

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