Radiation induced DNA damage and damage repair in three human tumour cell lines

Elies C. Woudstra*, Jeanette F. Brunsting, Judith M. Roesink, Antonius W. T. Konings, Harm H. Kampinga

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    23 Citations (Scopus)

    Abstract

    Three human tumour cell lines (HX142, RT112 and MGH-U1) with different radiosensitivities were tested for differences in the rate and/or extent of DNA unwinding in alkali as well as for differences in the induction of DNA double strand breaks by means of the pulsed field gel electrophoresis, after X-irradiation. Unlike that which has been found using the non-denaturing filter elution technique (NDE, McMillan et al., 1990), no differences in initial DNA damage (the extent of alkaline unwinding and the induction of double strand breaks) were found for the three cell lines. These data suggest that rather than a different number of DNA lesions per Da per Gy between these cell lines, structural differences in chromatin structure (related to radiosensitivity) might impair the detectability of lesions in some assays like the NDE. The nature of such structure differences remains unclear. However, the differences did not affect alkaline unwinding profiles, as all three cell lines showed identical rates of DNA unwinding after exposure to X-rays. Furthermore, the three cell lines did not show significant differences in the kinetics of DNA strand break rejoining nor in the amounts of damage remaining after 24 h repair. The results obtained in this study, together with other findings, suggest that the three cell lines may differ in their 'presentation' of DNA damage.

    Original languageEnglish
    Pages (from-to)51-59
    Number of pages9
    JournalMutation research-Dna repair
    Volume362
    Issue number1
    DOIs
    Publication statusPublished - 2-Jan-1996

    Keywords

    • alkaline unwinding
    • DNA strand breaks
    • CHEF
    • chromatin structure
    • pulsed field gel electrophoresis
    • radiation sensitivity
    • DOUBLE-STRAND BREAKS
    • IONIZING-RADIATION
    • CHEF ELECTROPHORESIS
    • CHROMATIN STRUCTURE
    • X-IRRADIATION
    • RADIOSENSITIVITY
    • INDUCTION
    • MUTANTS

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