Radioresistant pseudo-colony formation in the PHA-leukocyte feeder colony assay

J C Kluin-Nelemans, H W Hakvoort, S E Boom, E J Bast, R Willemze

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Abstract

In the PHA-leukocyte feeder colony assay--a fluid assay on top of an agar underlayer--colonies might not be the product of clonogenic cells but rather from aggregates, as was already shown for hairy cell leukemia (Leukemia Res. 11, 911 (1987)). To study the role of aggregation in this colony assay in other B-cell malignancies, we irradiated cells from B-chronic lymphocytic leukemia, B-non-Hodgkin's lymphoma and multiple myeloma. In nearly all cases, viable "colonies" were seen after irradiation, albeit in lower numbers. These data indicate that in the PHA-leukocyte feeder colony assay, a considerable percentage of colonies from a large variety of B-cell malignancies originate from aggregating rather than from proliferating cells.

Original languageEnglish
Pages (from-to)917-22
Number of pages6
JournalLeukemia Research
Volume12
Issue number11-12
Publication statusPublished - 1988
Externally publishedYes

Keywords

  • B-Lymphocytes
  • Colony-Forming Units Assay
  • Culture Media
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Leukemia, Prolymphocytic
  • Lymphoma
  • Lymphoma, Non-Hodgkin
  • Phytohemagglutinins
  • Tumor Cells, Cultured
  • Tumor Stem Cell Assay

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