Rapid Diagnosis of Azole-Resistant Aspergillosis by Direct PCR Using Tissue Specimens

Jan W. M. van der Linden; Eveline Snelders; Jan P. Arends; Simon M. Daenen; Willem J. G. Melchers; Paul E. Verweij

doi:10.1128/JCM.02221-09

Rapid Diagnosis of Azole-Resistant Aspergillosis by Direct PCR Using Tissue Specimens

Jan W. M. van der Linden
, Eveline Snelders
, Jan P. Arends
, Simon M. Daenen
, Willem J. G. Melchers
, Paul E. Verweij^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

51 Citations (Scopus)

Abstract

We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.

Original language	English
Pages (from-to)	1478-1480
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	4
DOIs	https://doi.org/10.1128/JCM.02221-09
Publication status	Published - Apr-2010

Keywords

NERVOUS-SYSTEM ASPERGILLOSIS
INVASIVE ASPERGILLOSIS
FUMIGATUS
VORICONAZOLE
MECHANISM
EFFICACY

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title = "Rapid Diagnosis of Azole-Resistant Aspergillosis by Direct PCR Using Tissue Specimens",

abstract = "We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.",

keywords = "NERVOUS-SYSTEM ASPERGILLOSIS, INVASIVE ASPERGILLOSIS, FUMIGATUS, VORICONAZOLE, MECHANISM, EFFICACY",

author = "\{van der Linden\}, \{Jan W. M.\} and Eveline Snelders and Arends, \{Jan P.\} and Daenen, \{Simon M.\} and Melchers, \{Willem J. G.\} and Verweij, \{Paul E.\}",

year = "2010",

month = apr,

doi = "10.1128/JCM.02221-09",

language = "English",

volume = "48",

pages = "1478--1480",

journal = "Journal of Clinical Microbiology",

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T1 - Rapid Diagnosis of Azole-Resistant Aspergillosis by Direct PCR Using Tissue Specimens

AU - van der Linden, Jan W. M.

AU - Snelders, Eveline

AU - Arends, Jan P.

AU - Daenen, Simon M.

AU - Melchers, Willem J. G.

AU - Verweij, Paul E.

PY - 2010/4

Y1 - 2010/4

N2 - We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.

AB - We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.

KW - NERVOUS-SYSTEM ASPERGILLOSIS

KW - INVASIVE ASPERGILLOSIS

KW - FUMIGATUS

KW - VORICONAZOLE

KW - MECHANISM

KW - EFFICACY

U2 - 10.1128/JCM.02221-09

DO - 10.1128/JCM.02221-09

M3 - Article

SN - 0095-1137

VL - 48

SP - 1478

EP - 1480

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Rapid Diagnosis of Azole-Resistant Aspergillosis by Direct PCR Using Tissue Specimens

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Keywords

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