Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method

Daniel Müller; Peter Hagedorn; Sabine Brast; Gerhard Heusipp; Martina Bielaszewska; Alexander W Friedrich; Helge Karch; M Alexander Schmidt

doi:10.1128/JCM.00895-06

Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method

Daniel Müller, Peter Hagedorn, Sabine Brast, Gerhard Heusipp, Martina Bielaszewska, Alexander W Friedrich, Helge Karch, M Alexander Schmidt

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

82 Downloads (Pure)

Abstract

Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

Original language	English
Pages (from-to)	2626-9
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00895-06
Publication status	Published - Jul-2006
Externally published	Yes

Keywords

DNA, Bacterial
Escherichia coli
Escherichia coli Infections
Feces
Genes, Bacterial
Humans
Polymerase Chain Reaction
Sensitivity and Specificity
Shiga Toxins

Access to Document

10.1128/JCM.00895-06

J. Clin. Microbiol.-2006-Müller-2626-9Final publisher's version, 75.9 KB

Handle.net

Persistent link

Cite this

Müller, D., Hagedorn, P., Brast, S., Heusipp, G., Bielaszewska, M., Friedrich, A. W., Karch, H., & Schmidt, M. A. (2006). Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method. Journal of Clinical Microbiology, 44(7), 2626-9. https://doi.org/10.1128/JCM.00895-06

@article{2b87f53ce8454b018f07605e970f9bab,

title = "Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method",

abstract = "Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.",

keywords = "DNA, Bacterial, Escherichia coli, Escherichia coli Infections, Feces, Genes, Bacterial, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Shiga Toxins",

author = "Daniel M{\"u}ller and Peter Hagedorn and Sabine Brast and Gerhard Heusipp and Martina Bielaszewska and Friedrich, {Alexander W} and Helge Karch and Schmidt, {M Alexander}",

year = "2006",

month = jul,

doi = "10.1128/JCM.00895-06",

language = "English",

volume = "44",

pages = "2626--9",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "AMER SOC MICROBIOLOGY",

number = "7",

}

Müller, D, Hagedorn, P, Brast, S, Heusipp, G, Bielaszewska, M, Friedrich, AW, Karch, H & Schmidt, MA 2006, 'Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method', Journal of Clinical Microbiology, vol. 44, no. 7, pp. 2626-9. https://doi.org/10.1128/JCM.00895-06

Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method. / Müller, Daniel; Hagedorn, Peter; Brast, Sabine et al.
In: Journal of Clinical Microbiology, Vol. 44, No. 7, 07.2006, p. 2626-9.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method

AU - Müller, Daniel

AU - Hagedorn, Peter

AU - Brast, Sabine

AU - Heusipp, Gerhard

AU - Bielaszewska, Martina

AU - Friedrich, Alexander W

AU - Karch, Helge

AU - Schmidt, M Alexander

PY - 2006/7

Y1 - 2006/7

N2 - Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

AB - Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

KW - DNA, Bacterial

KW - Escherichia coli

KW - Escherichia coli Infections

KW - Feces

KW - Genes, Bacterial

KW - Humans

KW - Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Shiga Toxins

U2 - 10.1128/JCM.00895-06

DO - 10.1128/JCM.00895-06

M3 - Article

C2 - 16825399

SN - 0095-1137

VL - 44

SP - 2626

EP - 2629

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Cite this