Rapid, variable aperture approach to quantify depth of fluorescence in a heterogenous medium

Cody C. Rounds, Jaron de Wit, Jasper Vonk, Floris Voskuil, Jennifer Vorjohan, Sophia Nelson, Brooke Villinski, Allyson Trang, Max J. H. Witjes, Kenneth M. Tichauer

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)


Post-operative assessment of resected tumor margins is critical to ensure the entirety of malignant tissue has been removed from a patient. Microscopic assessment of tissue post-excision is the current gold standard, however the long wait times for proper specimen evaluation limit a surgeon's ability to be certain they obtained clear margins. To address this need, fluorescence-guided surgery approaches are under development that can yield molecular contrast between healthy and malignant tissues intraoperatively. In head and neck cancer specifically, heterogenous optical properties lead to poor identification in margins greater than 1 mm thick when viewed with single projections. Thus, we demonstrate the use of variable aperture approach to decrease the effects of local optical property variations in the imaged specimen. Here we use Monte Carlo simulations to verify the utility of the idea in a homogenous medium as well in a medium with heterogenous properties. We demonstrate that a ratio metric approach can provide near identical depth discrimination as a single projection in a homogenous medium and is further capable of reducing pixel variability due to local optical properties in a heterogenous medium than a single projection alone.

Original languageEnglish
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationMolecules, Devices, and Applications IX
EditorsSylvain Gioux, Summer L. Gibbs, Brian W. Pogue
ISBN (Electronic)9781510658271
Publication statusPublished - 2023
EventMolecular-Guided Surgery: Molecules, Devices, and Applications IX 2023 - San Francisco, United States
Duration: 28-Jan-202329-Jan-2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMolecular-Guided Surgery: Molecules, Devices, and Applications IX 2023
Country/TerritoryUnited States
CitySan Francisco


  • Fluorescence-guided surgery
  • head and neck squamous cell carcinoma
  • intraoperative margin assessment
  • Monte Carlo simulations

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