TY - GEN
T1 - Real-Time, In-Line Monitoring of Oxygen-Dependent Metabolism of Mouse Precision-Cut Liver Slices Incubated in a Microfluidic Device
AU - Karsten, Ruby E.H.
AU - Grajewski, Maciej
AU - Mulder, Jean Paul S.H.
AU - Olinga, Peter
AU - Verpoorte, Elisabeth
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - An organ-on-a-chip (OOC) is a microfluidic system that allows the in-depth study of animal and human physiology, in an organ-specific ex vivo context. We developed an incubation system for precision-cut liver slices (PCLS) in which we actively modulate mitochondrial respiration of PCLS by alternating perfusion by regular medium with succinate-supplemented medium. The goal is to monitor dynamic responses in respiration in the same tissue sample in real time. Our data shows that we successfully monitored, and intervened with, mitochondrial respiration of PCLS. Moreover, we demonstrate that real-time monitoring of oxygen-dependent metabolism gives a more comprehensive description of the dynamic nature of this process than end-point analysis.
AB - An organ-on-a-chip (OOC) is a microfluidic system that allows the in-depth study of animal and human physiology, in an organ-specific ex vivo context. We developed an incubation system for precision-cut liver slices (PCLS) in which we actively modulate mitochondrial respiration of PCLS by alternating perfusion by regular medium with succinate-supplemented medium. The goal is to monitor dynamic responses in respiration in the same tissue sample in real time. Our data shows that we successfully monitored, and intervened with, mitochondrial respiration of PCLS. Moreover, we demonstrate that real-time monitoring of oxygen-dependent metabolism gives a more comprehensive description of the dynamic nature of this process than end-point analysis.
KW - Micromilling
KW - Mitochondria
KW - Optical-fiber-based oxygen sensing
KW - Organ-on-a-chip
UR - http://www.scopus.com/inward/record.url?scp=85136962713&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136962713
VL - 1
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 503
EP - 504
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -