Real-time visualization of intracellular hydrodynamics in single living cells

Eric O. Potma, Wim P. de Boeij, Peter J.M. van Haastert, Douwe A. Wiersma

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173 Citations (Scopus)

Abstract

Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics.
Original languageEnglish
Pages (from-to)1577 - 1582
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume98
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • DIFFUSIONAL WATER PERMEABILITY
  • NMR

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