Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?

Asel S. Arykbaeva; Lente J.S. Lerink; Jaël Vos; Marten A. Engelse; Cees van Kooten; Dirk de Korte; Johan W.M. Lagerberg; Thomas R.L. Klei; Aat A. Mulder; Robert C. Minnee; Rutger J. Ploeg; Cyril Moers; Robert A. Pol; Ian P.J. Alwayn; Dorottya K. de Vries; Jan H.N. Lindeman

doi:10.1016/j.ajt.2024.01.002

Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?

Asel S. Arykbaeva, Lente J.S. Lerink, Jaël Vos, Marten A. Engelse, Cees van Kooten, Dirk de Korte, Johan W.M. Lagerberg, Thomas R.L. Klei, Aat A. Mulder, Robert C. Minnee, Rutger J. Ploeg, Cyril Moers, Robert A. Pol, Ian P.J. Alwayn, Dorottya K. de Vries, Jan H.N. Lindeman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.

Original language	English
Number of pages	8
Journal	American Journal of Transplantation
DOIs	https://doi.org/10.1016/j.ajt.2024.01.002
Publication status	Accepted/In press - 2024

Keywords

kidney transplantation/nephrology
normothermic machine perfusion
organ perfusion and preservation
oxygen carrier
red blood cells

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Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts- Friend or foe?Final publisher's version, 1.1 MBLicence: CC BY

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Arykbaeva, A. S., Lerink, L. J. S., Vos, J., Engelse, M. A., van Kooten, C., de Korte, D., Lagerberg, J. W. M., Klei, T. R. L., Mulder, A. A., Minnee, R. C., Ploeg, R. J., Moers, C., Pol, R. A., Alwayn, I. P. J., de Vries, D. K., & Lindeman, J. H. N. (Accepted/In press). Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe? American Journal of Transplantation. https://doi.org/10.1016/j.ajt.2024.01.002

@article{5c9ee1ee626d44578981671ad2020c0f,

title = "Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?",

abstract = "Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.",

keywords = "kidney transplantation/nephrology, normothermic machine perfusion, organ perfusion and preservation, oxygen carrier, red blood cells",

author = "Arykbaeva, {Asel S.} and Lerink, {Lente J.S.} and Ja{\"e}l Vos and Engelse, {Marten A.} and {van Kooten}, Cees and {de Korte}, Dirk and Lagerberg, {Johan W.M.} and Klei, {Thomas R.L.} and Mulder, {Aat A.} and Minnee, {Robert C.} and Ploeg, {Rutger J.} and Cyril Moers and Pol, {Robert A.} and Alwayn, {Ian P.J.} and {de Vries}, {Dorottya K.} and Lindeman, {Jan H.N.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

year = "2024",

doi = "10.1016/j.ajt.2024.01.002",

language = "English",

journal = "American Journal of Transplantation",

issn = "1600-6135",

publisher = "Wiley",

}

Arykbaeva, AS, Lerink, LJS, Vos, J, Engelse, MA, van Kooten, C, de Korte, D, Lagerberg, JWM, Klei, TRL, Mulder, AA, Minnee, RC, Ploeg, RJ, Moers, C , Pol, RA, Alwayn, IPJ, de Vries, DK & Lindeman, JHN 2024, 'Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?', American Journal of Transplantation. https://doi.org/10.1016/j.ajt.2024.01.002

TY - JOUR

T1 - Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts

T2 - Friend or foe?

AU - Arykbaeva, Asel S.

AU - Lerink, Lente J.S.

AU - Vos, Jaël

AU - Engelse, Marten A.

AU - van Kooten, Cees

AU - de Korte, Dirk

AU - Lagerberg, Johan W.M.

AU - Klei, Thomas R.L.

AU - Mulder, Aat A.

AU - Minnee, Robert C.

AU - Ploeg, Rutger J.

AU - Moers, Cyril

AU - Pol, Robert A.

AU - Alwayn, Ian P.J.

AU - de Vries, Dorottya K.

AU - Lindeman, Jan H.N.

PY - 2024

Y1 - 2024

N2 - Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.

AB - Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.

KW - kidney transplantation/nephrology

KW - normothermic machine perfusion

KW - organ perfusion and preservation

KW - oxygen carrier

KW - red blood cells

UR - http://www.scopus.com/inward/record.url?scp=85182982002&partnerID=8YFLogxK

U2 - 10.1016/j.ajt.2024.01.002

DO - 10.1016/j.ajt.2024.01.002

M3 - Article

C2 - 38215981

AN - SCOPUS:85182982002

SN - 1600-6135

JO - American Journal of Transplantation

JF - American Journal of Transplantation

ER -

Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?

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Keywords

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