Reduced astrocyte density underlying brain volume reduction inactivity-based anorexia rats

Linda Frintrop*, Johanna Liesbrock, Lisa Paulukat, Sonja Johann, Martien J. Kas, Rene Tolba, Nicole Heussen, Joseph Neulen, Kerstin Konrad, Beate Herpertz-Dahlmann, Cordian Beyer, Jochen Seitz

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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OBJECTIVES: Severe grey and white matter volume reductions were found in patients with anorexia nervosa (AN) that were linked to neuropsychological deficits while their underlying pathophysiology remains unclear. For the first time, we analysed the cellular basis of brain volume changes in an animal model (activity-based anorexia, ABA).

METHODS: Female rats had 24 h/day running wheel access and received reduced food intake until a 25% weight reduction was reached and maintained for 2 weeks.

RESULTS: In ABA rats, the volumes of the cerebral cortex and corpus callosum were significantly reduced compared to controls by 6% and 9%, respectively. The number of GFAP-positive astrocytes in these regions decreased by 39% and 23%, total astrocyte-covered area by 83% and 63%. In neurons no changes were observed. The findings were complemented by a 60% and 49% reduction in astrocyte (GFAP) mRNA expression.

CONCLUSIONS: Volumetric brain changes in ABA animals mirror those in human AN patients. These alterations are associated with a reduction of GFAP-positive astrocytes as well as GFAP expression. Reduced astrocyte functioning could help explain neuronal dysfunctions leading to symptoms of rigidity and impaired learning. Astrocyte loss could constitute a new research target for understanding and treating semi-starvation and AN.

Original languageEnglish
Pages (from-to)225-235
Number of pages11
JournalThe World Journal of Biological Psychiatry
Issue number3
Publication statusPublished - 2018


  • ABA rat model
  • anorexia nervosa
  • astrocytes
  • corpus callosum
  • cortex volume

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