Reduced Life- and Healthspan in Mice Carrying a Mono-Allelic BubR1 MVA Mutation

Tobias Wijshake*, Liviu A. Malureanu, Darren J. Baker, Karthik B. Jeganathan, Bart van de Sluis, Jan M. van Deursen

*Corresponding author for this work

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Abstract

Mosaic Variegated Aneuploidy (MVA) syndrome is a rare autosomal recessive disorder characterized by inaccurate chromosome segregation and high rates of near-diploid aneuploidy. Children with MVA syndrome die at an early age, are cancer prone, and have progeroid features like facial dysmorphisms, short stature, and cataracts. The majority of MVA cases are linked to mutations in BUBR1, a mitotic checkpoint gene required for proper chromosome segregation. Affected patients either have bi-allelic BUBR1 mutations, with one allele harboring a missense mutation and the other a nonsense mutation, or mono-allelic BUBR1 mutations combined with allelic variants that yield low amounts of wild-type BubR1 protein. Parents of MVA patients that carry single allele mutations have mild mitotic defects, but whether they are at risk for any of the pathologies associated with MVA syndrome is unknown. To address this, we engineered a mouse model for the nonsense mutation 2211insGTTA (referred to as GTTA) found in MVA patients with bi-allelic BUBR1 mutations. Here we report that both the median and maximum lifespans of the resulting BubR1(+/GTTA) mice are significantly reduced. Furthermore, BubR1(+/GTTA) mice develop several aging-related phenotypes at an accelerated rate, including cataract formation, lordokyphosis, skeletal muscle wasting, impaired exercise ability, and fat loss. BubR1(+/GTTA) mice develop mild aneuploidies and show enhanced growth of carcinogen-induced tumors. Collectively, these data demonstrate that the BUBR1 GTTA mutation compromises longevity and healthspan, raising the interesting possibility that mono-allelic changes in BUBR1 might contribute to differences in aging rates in the general population.

Original languageEnglish
Article numbere1003138
Number of pages13
JournalPLoS genetics
Volume8
Issue number12
DOIs
Publication statusPublished - 27-Dec-2012

Keywords

  • MOSAIC VARIEGATED ANEUPLOIDY
  • PREMATURE CHROMATID SEPARATION
  • AGING-ASSOCIATED PHENOTYPES
  • MITOTIC-SPINDLE CHECKPOINT
  • CENTROMERE DIVISION PCD
  • AGE-OLD PROBLEM
  • CANCER PREDISPOSITION
  • CHROMOSOMAL INSTABILITY
  • MUTANT MICE
  • CONSTITUTIONAL ANEUPLOIDY

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