Regeneration of irradiated salivary glands with stem cell marker expressing cells

Lalitha S. Y. Nanduri; Martti Maimets; Sarah A. Pringle; Marianne van der Zwaag; Ronald P. van Os; Robert P. Coppes

doi:10.1016/j.radonc.2011.05.085

Regeneration of irradiated salivary glands with stem cell marker expressing cells

Lalitha S. Y. Nanduri
, Martti Maimets
, Sarah A. Pringle
, Marianne van der Zwaag
, Ronald P. van Os
, Robert P. Coppes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

156 Citations (Scopus)

Abstract

Background: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.

Methods: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.

Results: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.

Conclusions: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland. (C) 2011 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology 99 (2011) 367-372

Original language	English
Pages (from-to)	367-372
Number of pages	6
Journal	Radiotherapy and Oncology
Volume	99
Issue number	3
DOIs	https://doi.org/10.1016/j.radonc.2011.05.085
Publication status	Published - Jun-2011

Keywords

Salivary gland
Irradiation
Xerostomia
Stem cell transplantation
PROPHYLACTIC PILOCARPINE TREATMENT
SUBMANDIBULAR-GLANDS
PROGENITOR CELLS
DUCT
PROLIFERATION
AMELIORATION
GENERATION
LIGATION
PROSTATE
THERAPY

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Regeneration of irradiated salivary glands with stem cell marker expressing cells
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@article{85d57b47bce24ae696fd65e90e01f1df,

title = "Regeneration of irradiated salivary glands with stem cell marker expressing cells",

abstract = "Background: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.Methods: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.Results: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058\% vs. 0.65\%), CD133 (6\% vs. 5\%), CD49f (78\% vs. 51\%), and CD24 (60\% vs. 60\%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.Conclusions: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland. (C) 2011 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology 99 (2011) 367-372",

keywords = "Salivary gland, Irradiation, Xerostomia, Stem cell transplantation, PROPHYLACTIC PILOCARPINE TREATMENT, SUBMANDIBULAR-GLANDS, PROGENITOR CELLS, DUCT, PROLIFERATION, AMELIORATION, GENERATION, LIGATION, PROSTATE, THERAPY",

author = "Nanduri, \{Lalitha S. Y.\} and Martti Maimets and Pringle, \{Sarah A.\} and \{van der Zwaag\}, Marianne and \{van Os\}, \{Ronald P.\} and Coppes, \{Robert P.\}",

year = "2011",

month = jun,

doi = "10.1016/j.radonc.2011.05.085",

language = "English",

volume = "99",

pages = "367--372",

journal = "Radiotherapy and Oncology",

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publisher = "ELSEVIER IRELAND LTD",

number = "3",

}

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T1 - Regeneration of irradiated salivary glands with stem cell marker expressing cells

AU - Nanduri, Lalitha S. Y.

AU - Maimets, Martti

AU - Pringle, Sarah A.

AU - van der Zwaag, Marianne

AU - van Os, Ronald P.

AU - Coppes, Robert P.

PY - 2011/6

Y1 - 2011/6

N2 - Background: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.Methods: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.Results: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.Conclusions: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland. (C) 2011 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology 99 (2011) 367-372

AB - Background: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.Methods: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.Results: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.Conclusions: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland. (C) 2011 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology 99 (2011) 367-372

KW - Salivary gland

KW - Irradiation

KW - Xerostomia

KW - Stem cell transplantation

KW - PROPHYLACTIC PILOCARPINE TREATMENT

KW - SUBMANDIBULAR-GLANDS

KW - PROGENITOR CELLS

KW - DUCT

KW - PROLIFERATION

KW - AMELIORATION

KW - GENERATION

KW - LIGATION

KW - PROSTATE

KW - THERAPY

U2 - 10.1016/j.radonc.2011.05.085

DO - 10.1016/j.radonc.2011.05.085

M3 - Article

SN - 0167-8140

VL - 99

SP - 367

EP - 372

JO - Radiotherapy and Oncology

JF - Radiotherapy and Oncology

IS - 3

ER -

Regeneration of irradiated salivary glands with stem cell marker expressing cells

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