@inbook{7b44e3e1d27f4a448750812e02a850bc,
title = "Regulation of Antibiotic Production by Bacterial Hormones",
abstract = "Antibiotic production is regulated by numerous signals, including the so-called bacterial hormones found in antibiotic producing organisms such as Streptomyces. These signals, the γ-butyrolactones, are produced in very small quantities, which has hindered their structural elucidation and made it difficult to assess whether they are being produced. In this chapter, we describe a rapid small-scale extraction method from either solid or liquid cultures in scales of one plate or 50 ml of medium. Also described is a bioassay to detect the γ-butyrolactones by determining either the production of pigmented antibiotic of Streptomyces coelicolor or kanamycin resistant growth on addition of the γ-butyrolactones. We also describe some insights into the identification of the γ-butyrolactone receptor and its targets and also the gel retardation conditions with three differently labeled probes.",
keywords = "STREPTOMYCES-COELICOLOR A3(2), A-FACTOR RECEPTOR, BUTYROLACTONE AUTOREGULATOR, SECONDARY METABOLISM, GAMMA-BUTYROLACTONE, GENE-CLUSTER, BIOSYNTHESIS, PROTEIN, GRISEUS, FRI-5",
author = "Nai-Hua Hsiao and Marco Gottelt and Eriko Takano",
note = "Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",
year = "2009",
doi = "10.1016/S0076-6879(09)04806-X",
language = "English",
isbn = "978-0-12-374588-0",
series = "Methods in Enzymology",
publisher = "Academic Press",
pages = "143--157",
editor = "DA Hopwood",
booktitle = "COMPLEX ENZYMES IN MICROBIAL NATURAL PRODUCT BIOSYNTHESIS, PART A: OVERVIEW ARTICLES AND PEPTIDES",
}