Regulation of Rap1 signaling during Dictyostelium chemotaxis and development

Youtao Liu

Research output: ThesisThesis fully internal (DIV)

343 Downloads (Pure)

Abstract

Over the past two decades, Rap1 has been recognized world-wide to be an important regulator in a large variety of biological events, including chemotaxis. Despite substantial efforts, the regulation of Rap1 in chemotaxis is still not completely understood. In this thesis, four Rap1 activators, GflB, RasGefL, RasGefQ, and GbpD, have been discovered and characterized in amoeba Dictyostelium discoideum. Our results indicate that chemotactic receptors recruits Rap1 activators, especially GflB, to the sides facing the highest amount of chemical waves called “leading edge”, which then communicates the signal received from receptors to locally assemble the crawling machinery that orients movement toward the source. Although some mechanisms are still not fully unraveled, our findings provide a milestone in the understanding how cells decide on the direction in which they need to migrate towards the extracellular chemical cues. We hope that our work enlightens and encourages new ideas and further investigations on understanding this process.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • van Haastert, Peter, Supervisor
  • Kortholt, Arjan, Co-supervisor
  • van der Klei, Ida, Assessment committee
  • Schmidt, Martina, Assessment committee
  • Kops, G., Assessment committee, External person
Award date4-Nov-2016
Place of Publication[Groningen]
Publisher
Print ISBNs978-90-367-9143-4
Electronic ISBNs978-90-367-9141-0
Publication statusPublished - 2016

Cite this